摘要
目的:分析双藤痹痛凝胶对胶原免疫性关节炎大鼠外周CD_(4)^+CD_(25)^+FoxP3^+Treg细胞的调节作用。方法:Wistar大鼠50只,随机分成5组,分别为正常组、模型组、双藤痹痛凝胶高剂量组、中剂量组、低剂量组,胶原蛋白诱导大鼠关节炎,并采用流式细胞术检测大鼠外周血CD_(4)^+CD_(25)^+FoxP3^+Treg及酶联免疫吸附试验检测血清中IL-6表达水平。结果:与模型组比较,双藤痹痛凝胶高、低剂量组大鼠外周血CD_(4)^+CD_(25)^+T细胞及CD_(4)^+CD_(25)^+FoxP3^+Treg细胞水平显著升高,且高、中剂量组血清IL-6水平显著下降。结论:双藤痹痛凝胶治疗类风湿性关节炎可能通过上调外周血CD_(4)^+CD_(25)^+FoxP3^+Treg细胞表达水平,降低血清IL-6水平,从而恢复免疫耐受作用。
Objective To analyse the regulation effect of Shuangteng Bitong Cataplasm on CD4＋ CD2~ FoxP3 ＋ Treg Cell in Peripheral Blood from Rats with Collagen - Induced Arthritis （CIA）. Methods Fifty Wistar rats were randomly divided into 5 groups, normal control group, model control group, high - dose, middle - dose and low - dose Shuangteng Bitong Cataplasm group. Rats＂ arthritis was induced by collagen. The number of CD4＋ CD25 FoxP3 ＋ Treg cell in peripheral blood was tested by flow cytometry, and the level of IL - 6 in serum was detected by enzyme linked immunosorbent assay. Results Compared with the model groups, the levels of CD4 CD2~ FoxP3 ＋ Treg cell and CD4＋ CD~ FoxP3 ＋ Treg cell were increased obviously in the high and low dose of Shuangteng Bitong Cataplasm groups, while the level of IL - 6 in serum was decreased remarkably in the middle and high - dose of Shuangteng Bitong Cataplasm groups. Conclusion It is realized that Shuangteng Bitong Cataplasm effectively treat RA by regulating the level of Treg cell and reducing IL- 6 gene expression to restore immunological tolerance.
出处
《中国民族民间医药》
2016年第23期33-35,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
福建省科技厅引导性项目(2015Y0060)
