摘要
采用高速逆流色谱法从革皮氏海参中分离纯化得到皂苷单体Des-echinoside A,研究了Des-echinoside A对高转移人肺巨细胞癌95D细胞增殖和转移的影响及其分子机制。结果显示:Des-echinoside A能显著降低95D细胞增殖(P<0.01)、迁移(P<0.01)、侵袭(P<0.01)以及粘附能力(P<0.01);还能显著抑制鸡胚尿囊膜(chick embryo chorioallantoic membrane,CAM)上的血管新生。Des-echinoside A能显著降低u PA、MMP-2/-9、VEGF和NF-κB mRNA的表达(P<0.05,P<0.01),显著上调TIMP-1/-2 mRNA的表达(P<0.01),提示Desechinoside A可能通过调控NF-κB信号通路抑制95D细胞转移。
A triterpene glycoside Des-echinoside A was separated by high speed counter current chromatography from sea cucumber Pearsonothuria graeffei. The effects and molecular mechanism of Des-echinoside A on tumor metastasis in high-metastatic（ 95D） human lung cancer cells were evaluated. The results showed that Des-echinoside A could inhibit95 D cell proliferation（ P〈0. 01）,migration（ P〈0. 01）,invasion（ P〈0. 01）,adhesion（ P〈0. 01） and decrease the blood vessel growth on the chick embryo chorioallantoic membrane（ CAM） remarkably. Des-echinoside A could also reduce the mRNA expression of u PA,MMP-2/-9,VEGF,NF-κB（ P〈0. 05,P〈0. 01）,and promote the mRNA expression of TIMP-1/-2（ P〈0. 01）. It is suggested that Des-echinoside A could prevent the metastasis through specific inhibition of NF-κB signaling pathway.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第11期1691-1698,共8页
Natural Product Research and Development
基金
山东省自然科学基金(ZR2015PC002
ZR2014BP016)
鲁东大学人才引进基金(LY2012011)
关键词
皂苷
95D细胞
转移
血管新生
信号通路
triterpene glycoside
95D cell
metastasis
angiogenesis
signaling pathway
