摘要
背景:国内外研究表明,人羊膜间充质干细胞可分化为肝细胞样细胞,提示肝损伤后进行人羊膜间充质干细胞移植为临床肝病治疗提供可能。目的:观察人羊膜间充质干细胞移植对大鼠肝脏缺血-再灌注损伤的修复作用。方法:将60只SD大鼠随机分为3组,干细胞移植组、模型组建立大鼠肝脏缺血-再灌注损伤模型,造模后1 h,干细胞移植组于尾静脉注射人羊膜间充质干细胞0.5 mL(106个),模型组于尾静脉注射L-DMEM 0.5 mL,正常对照组于尾静脉注射生理盐水0.5 mL。移植后1,2,3周,进行肝功能、肝组织形态学、RT-PCR法及Western blot检测。结果与结论:(1)肝功能:与正常对照组比较,模型组移植后不同时间点的门冬氨酸转移酶、丙氨酸转移酶和丙二醛水平明显升高(P<0.05);与模型组比较,干细胞移植组移植后不同时间点的门冬氨酸转移酶、丙氨酸转移酶和丙二醛水平明显降低(P<0.05);(2)肝组织形态:移植后2周,模型组肝细胞变性、坏死及纤维化程度严重,干细胞移植组肝细胞变性、坏死及纤维化程度较模型组明显减轻;(3)RT-PCR法及Western blot检测:移植后2周,与模型组比较,干细胞移植组肝组织肝细胞生长因子水平明显升高(P<0.05),α-平滑肌蛋白明显下降(P<0.05);(4)结果表明:人羊膜间充质干细胞移植能有效改善大鼠受损的肝功能,其可能通过调节肝细胞生长因子及α-平滑肌蛋白在肝脏中的表达水平,进而发挥促进大鼠肝脏缺血-再灌注损伤修复的作用。
BACKGROUND：Studies have shown that human amniotic mesenchymal stem cel s can differentiate into hepatocyte-like cel s, suggesting that human amniotic mesenchymal stem cel transplantation provides a new potential for the clinical treatment of liver diseases. OBJECTIVE：To observe the effect of human amniotic mesenchymal stem cel transplantation on the repair of liver ischemia-reperfusion injury repair. METHODS：Sixty Sprague-Dawley rats were randomized into stem cel transplantation, model and control groups. Animal models of liver ischemia-reperfusion injury were made in the rats in the stem cel transplantation and model groups. One hour after modeling, rats in the stem cel transplantation were given injection of human amniotic mesenchymal stem cel s （0.5 mL, 106 cel s） via the tail vein, while rats in the model and control group were given L-DMEM （0.5 mL） or normal saline （0.5 mL）, respectively. Liver function and liver morphology were detected at 1, 2, 3 weeks after transplantation. Meanwhile, RT-PCR detection and western blot assay were also conducted. RESULTS AND CONCLUSION：（1） Liver function：Compared with the control group, levels of aspartate aminotransferase, alanine aminotransferase and malondialdehyde were significantly increased in the model group at different time points after transplantation （P〈0.05）, while a significant reduction in the levels of these three indicators was found after cel transplantation as compared with the model group （P〈0.05）. （2） Liver morphology：2 weeks after transplantation, rats in the model group exhibited hepatocyte degeneration and necrosis, and severe fibrosis, but these changes were remarkably al eviated in the stem cel transplantation group. （3） PT-PCR and western blot detection：2 weeks after transplantation, a significantly higher level of hepatocyte growth factor in the liver tissue and a lower level ofα-smooth muscle protein were found in the stem cel transplantation group compared with the model group （P〈0.05）.
出处
《中国组织工程研究》
CAS
北大核心
2016年第45期6788-6794,共7页
Chinese Journal of Tissue Engineering Research
