摘要
目的研究大鼠血管组织提取物于体外诱导骨髓间质干细胞(MSCs)向内皮细胞的分化能力。方法 2015年6—9月间使用淋巴细胞专用分离液对15例大鼠MSCs进行分离,以贴壁法行培养扩容,再采用流式细胞法检测MSCs的表层抗原水平,以基础培养液为实验A组,以含热处理的血管组织细胞提取物的培养液为实验B组,以含大鼠血管组织细胞提取物的培养液为实验C组,以含原代血管内皮组织细胞提取物的培养液作为实验D组,将第4代MSCs分别加入四组进行分化培养,分析对比细胞的表达变化,并检测内皮组织细胞于特异性标志物之变化表达。结果实验C组的MSCs的细胞形态未见显著改变,但细胞内的基因表达可见明显改变,内皮细胞的特异性基因CD144、CD34、CD31以及eNOS(endothelial nitric oxide synthase)出现高表达。结论血管组织细胞的提取物中含有能够诱导大鼠MSCs向内皮组织细胞分化的成份,提示成体细胞的内组成份对于相邻干细胞之分化结果具有至关重要的控制作用。
Objective To study the vascular tissue of rats to extract inducing bone marrow mesenchymal stem cells（MSCs） into endothelial cells differentiation. Methods From June to September 2015 using a separate liquid special for separation of 15 cases of lymphocytes in MSCs rats by adherent culture expansion, then the surface antigen level and flow cytometry was used to detect MSCs, in the basic culture medium as experimental group A, in cultured vascular tissue cell extracts containing heat treatment for the experimental group B in the medium containing vascular tissue, cell extracts of rats as experimental group C, with the medium containing primary vascular endothelial cells extract as experimental group D, the fourth generation of MSCs were added to the four group of differentiation and expression analysis of comparative cell change, and detect the endothelial cells on specific markers the change of expression.Results The cell morphology in experimental group C MSCs no significant changes, but the genes within the cell Expression of visible change obviously, endothelial cell specific gene CD144, CD34, CD31, and eNOS appear high expression. Conclusion Vascular tissue and cell extracts containing can induce rat MSCs to the composition of the endothelial cell differentiation, it was suggested that the somatic components to adjacent stem cell differentiation results have important role in controlling.
出处
《世界复合医学》
2016年第3期71-74,共4页
World Journal of Complex Medicine
关键词
骨髓间充质干细胞
组织细胞提取物
血管内皮细胞
分化
Bone marrow mesenchymal stem cells
Mesenchymal stem cells
Cell extracts
Endothelial cell differentiation
