摘要
目的探讨兔肠道组织抗菌有效成分的组成及其性质。方法将新鲜兔小肠组织匀浆,经高温处理,乙酸浸提后,检测抗菌活性,再经Sephadex G100和Sephadex G75凝胶柱过滤层析,收集具有抗菌活性的蛋白组分,经SDS-PAGE电泳,考马斯亮蓝染色后,为一条蛋白带,其相对分子质量约为43×103。用琼脂糖弥散法和活菌计数实验检测纯化物对9株细菌的抑菌作用。结果分离得到一种纯的兔肠源抗菌蛋白,纯化的兔肠源抗菌蛋白对9株细菌的均有明显的抑菌作用,杀菌率介于78%与98%之间,显示了较强的抗菌活性。结论初步显示了兔肠源抗菌蛋白在细菌性疫病的防治方面的应用前景。
Objective To study the nature and composition of an antimicrobial protein isolated from the small intestine of rabbit. Methods After the small intestine tissue of rabbit was treated by homogenation, heating and acetic acid extraction, antimicrobial components were isolated from the intestinal tissue of rabbit by Sephadex G100 and Sephadex G75 gel filtration chromatography. The samples were analyzed by SDS polyacrylamide gel electrophoresis. The antibacterial activity of the extract against 9 strains of bacteria were tested by agarose diffusion assay and live bacteria counting assay. Results The results showed that an antimicrobial protein was obtained and purified. Its molecule weight was about 43 ×10^3 . This antimicrobial protein strongly inhibited the 9 bacteria strains and the bacteria-killing rates varied from 78% to 98%. Conclusion An antimicrobial protein has been isolated and purified from the small intestine of rabbits. The strong inhibition effect of this protein on 9 bacteria strains indicates its promising potential application against bacterial diseases and it deserves further investigation.
出处
《中国实验动物学报》
CAS
CSCD
2007年第4期253-257,F0003,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金资助项目(编号:30400319)
关键词
兔
小肠
组织提取物
抗菌蛋白
抗菌活性
Rabbit
Small intestine
Tissue extract
Antibacterial protein
Antibacterial activity
