摘要
[目的]MYB蛋白家族是一类含有MYB保守结构域的转录因子,广泛参与调控植物生长发育及抗逆等多种生理反应。为探究抗旱作物谷子的抗旱性与MYB转录因子家族基因关系,从而为谷子特有抗旱基因资源的发掘及谷子抗旱分子机制的研究提供参考。[方法]本研究以抗旱品种勾勾母鸡咀(GG)和干旱敏感品种晋汾16(JF)为研究对象,在苗期PEG处理条件下,取叶片进行转录组测序,进而对有差异表达的MYB转录因子家族基因进行生物信息学分析。[结果]研究表明,26个MYB转录因子基因在GG和JF两个谷子品种中表现出较为明显的表达差异;且对应启动子区域的抗旱相关调控元件也呈现出差异的组成与分布;抗旱品种GG中明显上调的3个MYB调控基因(Si034363m、Si003539m、Si030711m)与拟南芥中主要参与抗逆的MYB转录因子存在较近亲缘关系。[结论]基于对谷子MYB转录因子的研究,推测26个表达差异的MYB类转录因子基因可作为谷子抗旱的重要候选基因,可为后续谷子抗旱分子机制的研究提供理论依据。
[Objective]MYB transcription factors are a class of protein with conserved MYB domain.They are involved in the regulation of plant growth,stress resistance and other physiological responses.Foxtail millet is an important drought-tolerance crop in northern China.It is important to explore the unique genetic resources and the molecular mechanism of drought-tolerance in foxtail millet to understand the relationship between drought resistance and MYB transcription factor family genes.[Methods]In this study,the drought-tolerant variety Go Go Mu Ji Ju（GG）and the drought sensitive variety Jin Fen 16（JF）were used as experimental materials,they were treated by PEG6000 in seedling stage,and transcriptome sequencing was conducted with mRNA from GG and JF leaves,bioinformatics analysis were used to analyze the differentially expressed MYB transcription factors.[Results]The results showed that：there were 26 MYB transcription factor genes that showed apparent differential expression in GG and JF,in addition,the drought-tolerant regulatory elements in promoters of the corresponding genes showed different composition and distribution.Si034363 m,Si003539m and Si030711 m,which were up-regulated upon drought stress in GG,showed close homology with some MYB transcription factors that mainly involved in drought tolerance in Arabidopsis.[Conclusion]Based on the research of MYB transcription factors in foxtail millet,we speculated that 26 differentially expressed MYB transcription factor genes could be used as important drought tolerant candidate genes in foxtail millet.This study could provide a theoretical basis for the study of the molecular mechanism of drought tolerance in foxtail millet.
出处
《山西农业大学学报（自然科学版）》
CAS
2016年第12期846-849,867,共5页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金(31471556)
关键词
谷子
MYB转录因子
干旱胁迫
生物信息学分析
Setaria italica
MYB transcription factors
Drought stress
Bioinformatics analyses
