摘要
目的以施万细胞作为周围神经模型，比较富血小板纤维蛋白（PRF）与浓缩生长因子（CGF）对其的影响。方法随机选取18-55岁身体健康志愿者10人，无菌条件下采集静脉血10mL，制备PRF和CGF。将细胞随机分为对照组、PRF组和CGF组。倒置相差显微镜下观察细胞形态；MTF比色法测定细胞增殖能力；酶联免疫吸附法检测细胞上清液中神经生长因子的分泌量；流式细胞仪检测细胞周期。结果倒置相差显微镜下观察各组细胞形态无明显差异；MTT结果显示PRF组和CGF组与对照组相比光密度值明显升高（P〈0．05），细胞上清液中神经生长因子分泌量均明显升高（P〈0．05），处于S＋G2M期的细胞数明显增多（P〈0．01），而PRF组与CGF组相比各项指标的差异无统计学意义。结论PRF和CGF均可以促进施万细胞增殖并增加神经生长因子的分泌量，但就改善细胞增殖能力、促进神经生长因子分泌来看，二者无明显差异。
Objective To compare the effects of platelet rich fibrin （PRF） and concentrated growth factors （CGF） using Schwann cells as a peripheral nerve model. Methods A total of 10 healthy volunteers aged 18 to 55 were randomly selected, and 10 mL venous blood was collected under aseptic conditions to prepare PRF and CGF. The cells were randomly divided into three groups, control group, PRF group and CGF group. The cell morphology was observed by inverted phase contrast microscope. The cell proliferation was analyzed by M3T assay. The secretion of nerve growth factor in supernatant was detected by enzyme-linked immunosorbent assay. Cell cycle was detected by flow cytometry. Results There was no significant difference of the morphology of cells in each group as observed under inverted phase contrast microscope. MTF results showed that the absorbance values of PRF group and CGF group were significantly higher than those of the control group （P 〈 0.05）. The secretion of nerve growth factor in the supernatant were significantly increased （P 〈 0.05 ）. The number of cells in S ＋ G2M phase was significantly increased （P 〈 0.01 ）, but there was no significant difference between PRF group and CGF group. Conclusion Both PRF and CGF can promote proliferation of Schwann cells and increased the amount of nerve growth factor secretion, but there is no significant difference between PRF and CGF in terms of improving cell proliferation and promoting nerve growth factor secretion.
作者
吴晶洋
白艳洁
王稚英
WU Jingyang BAI Yanjie WANG Zhiying(Center of Dental Implant, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China Department of Stomatology, Fushun Hospital, Shengiing Hospital Group of China Medical University, Fushun 113000, China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2016年第12期1089-1093,共5页
Journal of China Medical University
基金
辽宁省科技厅优秀人才培育项目(2014022025)
