摘要
目的：探讨前B细胞克隆增强因子（PBEF）在肥胖大鼠急性呼吸窘迫综合征（ARDS）中的变化及其可能作用。方法将40只健康雄性SD大鼠按随机数字表法分为对照组、肥胖组、ARDS组和肥胖ARDS组，每组10只。肥胖两组大鼠采用高脂饲料喂养，另两组喂养普通饲料；待高脂饲养大鼠体重超过正常饲养大鼠20%时为肥胖模型建立成功。此时ARDS两组大鼠经尾静脉注射脂多糖（LPS）构建ARDS模型，另两组则注射等量生理盐水。于ARDS制模后8h开胸取肺组织，测定肺干/湿重（D/W）比值；采用实时荧光定量反转录-聚合酶链反应（RT-PCR）检测PBEFmRNA表达；用蛋白质免疫印迹试验（WesternBlot）检测PBEF、核转录因子-κBp50（NF-κBp50）蛋白表达；采用免疫组化法检测PBEF阳性表达；苏木素-伊红（HE）染色后光镜下观察肺组织病理学改变。结果高脂饲料喂养第6周成功建立肥胖大鼠模型。肥胖组、ARDS组、肥胖ARDS组肺D/W比值明显低于对照组（0.195±0.005、0.179±0.003、0.153±0.011比0.224±0.007，均P＜0.05），且肥胖ARDS组显著低于ARDS组，说明肥胖对非ARDS及ARDS大鼠的肺含水量均有影响。肥胖组、ARDS组、肥胖ARDS组肺组织PBEFmRNA表达量依次升高〔分别为对照组的（1.77±0.22）、（3.29±0.14）、（5.52±0.14）倍，均P＜0.01〕，肺组织PBEF、NF-κBp50蛋白表达量也依次升高〔PBEF分别为对照组的（1.75±0.16）、（2.71±0.19）、（3.83±0.18）倍，NF-κBp50分别为对照组的（1.56±0.12）、（1.95±0.12）、（2.48±0.24）倍，均P＜0.01〕，且各组间两两比较差异均有统计学意义。免疫组化显示，对照组肺组织中几乎无PBEF阳性表达；肥胖组有少量PBEF阳性表达；ARDS组PBEF阳性表达明显增多；肥胖ARDS组肺泡壁、肺泡腔内的渗出液中有强阳性反应。HE染色显示，对照组肺组织结构正常；肥胖组有少许炎性细胞浸润；ARDS组肺�
Objective To investigate the variation and potential function of pre-B cell colony enhancing factor （PBEF） in obesity rats with acute respiratory distress syndrome （ARDS）. Methods Forty healthy male Sprague-Dawley （SD） rats were randomly divided into control group, obesity group, ARDS group, and obesity ARDS group, with 10 rats in each group. Rats in the two obesity groups were fed with high-fat diet, and the rats in other groups were fed with normal fodder. After successful obesity models were reproduced as the mean weight of rats in obesity model groups was up over 20% compared to other groups, the rats in two ARDS groups were injected with lipopolysaccharide （LPS） through tail vein to reproduce ARDS models, and the rats in other groups were injected with the same volume of saline. The lung tissues were harvested at 8 hours after ARDS model reproduction to determine lung dry/wet weight （D/W） ratio; real-time fluorescence quantitative reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of PBEF mRNA, and the proteins expressions of PBEF and nuclear factor-κB p50 （NF-κB p50） were detected by Western Blot; the distribution and expression of PBEF was also examined by immunohistochemical staining. Pathological changes of lung tissues were observed after hcmatoxylin-eosin （HE） staining. Results Obesity rat models were set up successfully with 6 weeks of high fat diet. The D/W ratio of rats in obesity, ARDS, and obesity ARDS groups was significantly lower than that of control group （0.195±0.005, 0.179±0.003, 0.153±0.011 vs. 0.224±0.007, all P 〈 0.05）, and D/W ratio in obesity ARDS group was significantly lower than that of ARDS group, indicating that the lung water content of ARDS rats and non ARDS rats could be influenced by obesity. In obesity group, ARDS group and obesity ARDS group, the expression of PBEF mRNA in lung tissues was increased in turn [it was （1.77±0.22）, （3.29±0.14）, （5.52±0.14） folds of that in control g
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2016年第11期983-987,共5页
Chinese Critical Care Medicine
基金
重庆市卫生局医学科研计划项目（X1355）
关键词
肥胖
急性呼吸窘迫综合征
前B细胞克隆增强因子
Obesity
Acute respiratory distress syndrome
Pre-B cell colony enhancing factor
Nuclear factor-κB
