摘要
目的 探讨二烯丙基三硫（DATS）对脂多糖（LPS）致急性肺损伤（ALI）小鼠肿瘤坏死因子-α（TNF-α）表达及核转录因子-kB（NF—kB）活性的影响。方法 复制LPS致ALI小鼠模型。实验动物按随机数字表法分为生理盐水对照组、ALI模型组、DATS预防组、DATS治疗组和DATS对照组。采用酶联免疫吸附法（ELLSA）测定各组血清和肺组织匀浆上清液中TNF—α浓度；用逆转录-聚合酶链反应（RT—PCR）检测各组肺组织TNF-α mRNA表达；凝胶电泳迁移率改变分析（EMSA）检测肺组织NF—kB活性。结果 ALI模型组2h血清及肺组织TNF—α含量明显升高（P均〈0．01），6h有所降低，但仍高于生理盐水和DATS对照组（P均〈0．01）；DATS预防组2h和6h血清及肺组织TNF—α含量较ALI模型组均明显降低（P〈0．05或P＜0．01），但DATS治疗组无明显效果（P均〉0．05）。ALI模型组2h肺组织TNF—α mRNA表达较生理盐水对照组和DATS对照组均明显升高（P均〈0．01），DATS预防组可明显抑制肺组织中TNF—α mRNA表达（P〈0．05），但DATS治疗组无明显效果。ALI模型组肺组织NF～kB活性较生理盐水对照组和DATS对照组均明显升高（P均〈0．05），DATS预防组可明显抑制肺组织NF—kB活性（P〈0．05），但DATS治疗组的抑制效果不明显。结论 预先给予DATS可抑制LPS诱导的ALI小鼠肺组织NF—kB活性和TNF—α mRNA表达，减少血清及肺组织中TNF—α的生成，具有一定的抗ALI作用。
Objective To investigate the effect of diallyl trisulfide （DATS） on tumor necrosis factor- α （TNF- α） expression and nuclear factor -kB （NF- kB） activity in mice with acute lung injury （ALI） induced by lipopolysaccharide （LPS）. Methods ALI murine model was reproduced by injection of LPS intraperitoneally. Mice were randomly divided into normal saline control group, ALI group, DATS prevention group, DATS treatment group, and DATS control group. The TNF - α levels in the serum and in the supernatant of lung homogenates were measured with enzyme linked immunoadsorbent assay （ELISA）. The expression of TNF - α mRNA in the lung tissues was detected by reverse transcription polymerase chain reaction （RT - PCR）. NF - kB activity in the lung tissues was detected by electrophoresis mobility shift assay （EMSA）. Results The levels of TNF - α induced by LPS in the serum and the supernatant of lung homogenates were increased markedly at 2 hours in ALI group （both P〈0.01）, and decreased at 6 hours, but they were still higher than those of the control groups （all P〈0.01）. They were reduced in DATS prevention group at 2 and 6 hours compared with those of ALI group （P〈0.05 or P〈0.01）, but no change was noted in DATS treatment group （all P〉0.05）. The expression of TNF - α mRNA in the lung tissues of ALI group increased markedly at 2 hours compared with those of control groups （both P〈0. 01）, and it could be down- regulated by pretreatment with DATS （P〈 0. 05）. No change in DATS was found in treatment group. NF - kB activity in the lung tissue increased in ALI group compared with that of control groups （both P〈0.05）, and it was markedly reduced in DATS prevention group （P〈0. 05）, but no change was found in DATS treatment group. Conclusion Pretreatment of DATS for ALI in mice could inhibit NF-kB activaty, TNF - α mRNA expression in lung tissues, and decrease the release of TNF - α in the serum and the lung homogenates, and they might be the unde
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2007年第4期205-208,共4页
Chinese Critical Care Medicine
基金
河北省科技厅基金资助项目（05276101D-65）
