摘要
目的:探讨肉苁蓉对气管切开插管大鼠肺部病理及肺泡灌洗液(BALF)肺泡表面活性物质相关蛋白A(SPA)的影响。方法:将54只SD雄性大鼠采用随机数字表法分为A组(正常组18只)、B组(气管切开插管模型组18只)、C组(气管切开插管+肉苁蓉干预18只)。各组分别于24 h、72 h、168 h 3个时段取材6只。取材时结扎并取右肺组织行病理切片检查,采用Smith评分半定量分析;于左肺取肺泡灌洗液,应用SP-A ELISA试剂盒检测SP-A浓度。结果:肺组织病理切片显示B组、C组大鼠24 h、72 h、168 h时段均有不同程度的水肿、肺泡及间质炎症、出血、肺不张等肺组织损伤,Smith评分高于对应时段正常大鼠。C组3个时段评分与B组对应时段评分比较无明显差异。气管切开插管大鼠24 h、72 h、168 h 3个时段肺组织损伤程度不同,随着时间增加损伤程度不断加重,3个时段有明显差异。B组、C组大鼠肺泡灌洗液SP-A浓度在24 h、72 h、168 h 3个时段均明显高于对应时段空白对照组,以24 h浓度最高,168 h最低,呈随气管切开插管时间的延长而浓度不断降低的趋势,C组168h SP-A浓度高于B组。结论:气管切开插管可导致水肿、肺泡炎症、出血、肺不张等肺组织损伤及肺部固有免疫功能紊乱,SP-A有保护肺组织作用,早期SP-A代偿性增高,随后逐渐下降,而肉苁蓉在后期可提高SP-A的浓度而抑制肺组织的损害。
Objective：To observe the effects of Herba Cistanches on lung tissue pathology and SP - A in BALF in rats with tracheostomy tube. Methods：All of 54 male SD rats were randomly divided into 3 groups：normal control（ A）, model with tracheostomy tube（B） ,the herba cistanches - treatment（C）. Each 6 rats of each group were killed after 24 h ,72 h, 168 h and the lung tissues were sent to take pathological examination immediately. Smith scores were used to evaluate the injuries. The concentrations of SP - A in BALF were detected with ELISA method. Results ： The observation of lung tissue pathology was found normal in group A and injuries such as edema, alveolar and interstitial inflammation, alveolar and interstitial hemorrhage, atelectasis were observed in group B and C. The Smith scores in group B and C were higher than group A. The injuries of lung tissues examined at three phases （24 h,72 h,168 h） were different in group B and C. They became severer as the time went on. The concentrations of SP - A in BALF in group B and C were significantly higher than group A at 24 h,72 h and 168 h and highest at 24 h,lowest at 168 h,they became more and more lower as the time went on. Conclusion：The tracheostomy tube on rats may lead to lung injuries, such as edema, alveolar and interstitial inflammation, alveolar and interstitial hemorrhage, atelectasis and also lead to immunologic derangement. The SP - A shows its protective function and its level in BALF increases first and then gradually decreases. The herba cistanches can protect lung tissue through improving the concentration of SP - A.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第11期2436-2438,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81460687)
广西壮族自治区卫生厅中医药科技专项(GZPT13-35)
