摘要
目的探讨外周血淋巴细胞亚群在妊娠合并脓毒血症患者中的变化及其对判断患者疾病严重程度的意义。方法选择2010年1月至2015年12月58例妊娠合并脓毒症患者（妊娠合并脓毒症组），同时选取62例同期健康妊娠者（妊娠组）和60例健康育龄期女性（对照组）。所有受试人员采集空腹静脉血2ml，经流式细胞仪检测淋巴细胞亚群。分析各组患者外周血淋巴细胞亚群与疾病严重程度的关系。结果妊娠合并脓毒症组CD4＋T淋巴细胞、NK细胞、CD4＋／CD8＋较妊娠组和对照组显著降低，而脓毒症组中发生脓毒症休克组较未发生脓毒症休克组CD4＋T淋巴细胞、NK细胞及CD4＋／CD8＋明显进行性下降。妊娠组CD4＋T淋巴细胞、NK细胞和CD4＋／CD8＋较健康育龄期未孕组显著降低；妊娠合并脓毒症组恢复期CD4＋T淋巴细胞、NK细胞和CD4＋/CD8＋比例较急性期明显升高。结论妊娠期母体细胞免疫处于抑制状态，合并脓毒症时细胞免疫功进一步受损，导致严重脓毒血症，若CD4＋T淋巴细胞、NK细胞及CD4＋／CD8＋比例显著降低预示该脓毒症孕妇病情重，容易发展成脓毒症休克。
Objective To evaluation the disease severity on pregnancy combined with sepsis by lymphocyte subset of peripheral blood cells. Methods 58 case pregnancy with sepsis （ pregnancy with sepsis group ） , 62 case pregnancy （ pregnancy group ） and 60 case female in children bearing period （ control group ） were selected from January, 2010 to December, 2015 in First People＇ s Hospital of Hangzhou. In all of them, peripheral blood lymphocytes subset were examined by FCM and disease severity （ shock and non-shock ） was evaluated, and the relationship of peripheral blood lymphocytes subset and disease severity was analyzed. Results The CD4＋T cells, NK cells and the CD4＋/CD8＋ ratio were significantly decreased in pregnancy with sepsis group compared with pregnancy group. The CD4＋T cells, NK cells and the CD4＋/CD8＋ ratio were significantly decreased in pregnancy with sepsis group with shock compared with those of non-shock.The CD4＋T cells, NK cells and the CD4＋/CD8＋ ratio were significantly decreased in pregnancy group compared with control group. Conclusions Immune inhibition was happened in female with pregnancy, and furthered decreased with sepsis, which led to more severe sepsis.CD4＋T cells, NK cells and CD4＋/CD8＋ were predict factor of disease severity of pregnancy with sepsis.
出处
《浙江临床医学》
2016年第12期2319-2320,共2页
Zhejiang Clinical Medical Journal
