摘要
以平邑甜茶实生苗为试材,采用^(15)N同位素示踪法、气压过程分离法(BaPS)和磷酸甘油双层海绵通气法,研究酰胺态有机氮[CO(NH_2)_2-N]、铵态氮(NH_4^+-N)及硝态氮(NO_3^--N)对平邑甜茶^(15)N利用、分配和损失的影响。结果表明:5月17日施无机氮肥对植株生物量积累效果显著,铵态氮和硝态氮^(15)N利用率分别为13.68%和13.25%,显著高于有机氮肥的5.25%;早施氮肥还可有效抑制氮素的土壤损失,其中施NO_3^--N处理植株的氨挥发损失仅占施氮量的1.83%;晚施(7月15日)处理中,无机氮肥利用率较早期施用显著减少时对酰胺态有机氮利用率和氨挥发损失影响不大。所以早期施用NH_4^+-N或NO_3^--N是确保植株生长量和氮肥利用率的有效措施,后期合理配施酰胺态有机氮则是减少氮肥损失的有效途径。
In this experiment, we used one-year-old apple rootstock (Malus hupehensis Rehd.) as materials and 15N labeled tracer technique as tool, to investigate the effect of using each nitrogen forms in different time by the index of N utilization and loss. The results showed that in the treatment of 17 May, the biomass of plant, which was treated with inorganic nitrogen (NH4+-N and NO3--N), was obviously higher than the others. Under the treatment of ammonium nitrogen and nitrate nitrogen, the 15N utilization rate of plant reached its maximum 13.68% and 13.25%, which were significantly higher than the 5.25% of organic nitrogen. The loss of nitrification and ammonia volatilization was also stay low, such as the loss of NO3--N through ammonia volatilization, was only account for 1.83% of the nitrogen applied. Under the treatment of 15 July, the utilization ratio of inorganic nitrogen decreased significantly, but it had hardly effect on the index of utilization ratio and ammonia volatilization loss under the treatment of organic nitrogen. Therefore, using NH4+-N and NO3-N in 17 May is an effective measure to ensure the growth of plant and high level of nitrogen utilization, and when they are applied in 15 July, reasonable collocation of organic nitrogen can reduce the loss of nitrogen efficiently.
出处
《园艺学报》
CAS
CSCD
北大核心
2016年第11期2223-2232,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31501713)
国家重点研发计划项目(2016YFD0201100)
国家现代农业产业技术体系建设专项资金项目(CARS-28)