摘要
梅花鹿养殖是近年来我国南方地区出现的新兴养殖业.为探究梅花鹿养殖对养殖环境抗生素抗性基因的影响,采用高通量荧光定量PCR研究了抗生素抗性基因在养殖场区对照土壤、梅花鹿新鲜粪便、梅花鹿粪便堆肥产物和施用堆肥菜地土壤中的分布、丰度和多样性.结果表明,抗性基因的绝对丰度梅花鹿粪便堆肥产物>梅花鹿新鲜粪便>施用堆肥菜地土壤>养殖场区对照土壤,抗生素抗性基因在4种环境样品中具有不同分布格局;梅花鹿养殖场环境中抗性基因丰度与可移动基因元件丰度显著相关(P<0.05),表明可移动基因元件可能加快了抗性基因的水平转移过程,促进了抗性基因的迁移、传播和富集,加剧了养殖环境抗生素抗性基因污染.
Sika deer breeding is rapidly developing in South China during the recent years ; however, the impact of sika deer breeding on the abundance and diversity of antibiotic resistance genes （ARGs） in sika deer farm remains elusive. In this study, profiles of ARGs were investigated using high-throughput qPCR technique. The results showed that the abundance of ARGs varied among different samples in the following order, deer manure composting 〉 deer manure 〉 vegetable soil 〉 pristine soil, and the profiles of ARGs in these samples were significantly different （ P 〈 0.05 ）. The abundance of ARGs was significantly correlated to that of mobile genetic elements （MGEs） , implicating the potential of horizontal transfer of ARGs, which might accelerate the dissemination and enrichment of ARGs in the environment of sika deer farm.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2016年第11期4402-4409,共8页
Environmental Science
基金
国家重点研发计划重点专项(2016YFD0800205)
国家自然科学基金项目(31270153)
国家自然科学基金国际(地区)合作交流项目(21210008)
关键词
梅花鹿
养殖环境
抗生素
抗生素抗性基因
高通量定量PCR
sika deer
farm-associated environment
antibiotic resistance
antibiotic resistance genes
high-throughput qPCR
