摘要
采用平板显色初筛、Underbofler滴定法复筛及紫外-硫酸二乙酯复合诱变的方法,从临沂果园土样中得到一株产葡萄糖氧化酶(GOD)活性较好的菌株,命名为Bla-6,其产GOD酶活为(10.50±0.25)U/mL,与原始菌株LPA-4产GOD酶活(6.75 U/mL)相比提高了55.57%。结合5.8S rDNA-ITS区序列系统进化、菌落形态、生长特性、菌体形态等确定其分类地位。结果表明,所得菌株Bla-6被鉴定为黑曲霉(Aspergillus niger),经8代传代培养后,GOD酶活维持在10.5 U/mL左右,该菌株遗传稳定性较好。
By preliminary screening of plate coloration, secondary screening of Underbofler titration method, a glucose oxidase-producing strain was obtained from Linyi orchard soil samples, and named as strain Bla-6. The glucose oxidase （GOD） activity produced by strain Bla-6 was （10.50＋0.25） U/ml, which was 55.57% higher than that （6.75 U/ml） produced by original strain LPA-4. By the analysis of 5.8S rDNA-1TS gene sequence, colo- nial morphology, growth characteristics, and mycelial morphology, the results showed that the strain Bla-6 was identified as Aspergillus niger. Aider subculture of 10 successive generations, the GOD activity maintained at about 10.5 U/ml, which showed that the strain had good genetic stability.
作者
辛国芹
董佩佩
汪祥燕
赵影
刘秀侠
徐海燕
谷巍
XIN Guoqin DONG Peipei WANG Xiangyan ZHAO Ying LIU Xiuxia XU Haiyan GU Wei(Shandong Baolai-leelai Bioengineenng Co., Ltd., Taian 271000, China)
出处
《中国酿造》
CAS
北大核心
2016年第11期69-72,共4页
China Brewing
基金
国家高技术研究发展计划‘863计划’子课题(2013AA102806)
关键词
葡萄糖氧化酶
黑曲霉
诱变
遗传稳定性
glucose oxidase
Aspergillus niger
mutation
genetic stability
