摘要
目的建立黔产宽叶缬草Valeriana officinalis L.var.latifolia Miq挥发油的质量标准。方法 TLC法进行定性鉴别,GC法定量测定乙酸龙脑酯含有量。结果挥发油透明,显淡黄色,具有其固有的气味和滋味,22批样品的平均酸值为3.49 mg/g,平均皂化值为178.71 mg/g,平均折光率为1.476 7,平均比旋度为49.17°。TLC斑点清晰,专属性强。乙酸龙脑酯在1.015 9～8.020 0 mg/m L范围内线性关系良好,平均加样回收率为98.45%（RSD=1.01%）。结论该方法重复性好,专属性强,可用于黔产宽叶缬草挥发油的质量控制。
AIM To establish the quality standard for volatile oil from Valeriana officinalis L. var. latifolia Miq cultivated in Guizhou. METHODS TLC was used for the qualitative identification. GC was applied to quantitatively determining the borneol acetate content. RESULTS The volatile oil,with an inherent flavor and fragrance,was transparent,yet light yellow. The average acid value of twenty-two batches of samples was verified to be 3. 49 mg / g,the average saponification value was 178. 71 mg / g,the average refractive index was 1. 476 7,and the average specific rotation was 49. 17°. The clear spots demonstrated strong specificity of TLC method. Borneol acetate displayed a good linear relationship within the range of 1. 015 9-8. 020 0 mg / m L,whose average recovery was 98. 45%（ RSD = 1. 01%）. CONCLUSION This reproducible and specific method can be used for the quality control of volatile oil from V. officinalis cultivated in Guizhou.
出处
《中成药》
CAS
CSCD
北大核心
2016年第11期2400-2404,共5页
Chinese Traditional Patent Medicine
基金
贵州省高层次人才特助经费项目(黔科合LG字[2012]013)
关键词
宽叶缬草
挥发油
乙酸龙脑酯
TLC
GC
Valeriana officinalis L.var.latifolia Miq
volatile oil
borneol acetate
TLC
GC
