摘要
本文以PEG-柠檬酸钠双水相体系为研究对象,研究了外加电场对聚合物-有机盐双水相体系分相速率的影响。结果显示增加电场可使聚合物-有机盐双水相体系分相速率提高1-3倍。进而考察了外加电场方向、双水相体系的系线长度（TLL）、相比（VR）、PEG分子量对双水相体系分相速率和电促分相效率的影响。结果表明,电场方向对电促分相效率影响不大;系线长度对分相速率无明显规律性影响,而对电促分相效率影响较大,TLL=40时电促分相效率最高;随着双水相体系相比或成相组分PEG分子量的增加,双水相体系分相速率增大,而电促分相效率降低。
Electrokineticdemixing of PEG-organic salt aqueous two-phase system was studied in this paper.PEG-sodium citrate was selected as the model system.It was shown that added electric field could effectively accelerate the demixing rate of two-phase systems 1-3 times.Then the effect of field polarity of added field,volume ratio（ VR） of top and bottom phase,tie line length（ TLL） of aqueous two-phase system and PEG molecular weight on demixing rate and on electrokineticdemixing efficiency were taken into study.It turned out that the field polarity had little effect on electrokineticdemixing efficiency; TLL made no obvious regular effect on demixing rate,but had great influence on electrokineticdemixing efficiency. When TLL = 40,theaqueous two-phase system had the highest electrokineticdemixing efficiency; the demixing rate went up and the electrokineticdemixing efficiency went down as the aqueous two-phase system phase ratio increasing or the phase composition PEG molecular weight growing.
作者
吴学艳
刘杨
WU Xue -yan L IU Yang(Department of Biology, College of Science, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University,Shantou 515063,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2016年第11期1533-1539,共7页
Chemical Research and Application
基金
国家自然科学基金项目(21476135)资助
广东省高等学校优秀青年教师培养计划项目(Yq2013076)资助
汕头市科技计划项目(46)资助
关键词
双水相体系
电促分相
电场方向
系线长度
相比
aqueous two-phase system
electrokineticdemixing
field polarity
tie line length
volume ratio
