摘要
目的建立静态顶空进样-气相色谱-质谱(SHS-GC-MS)联用技术结合保留指数(RI)分析沉香香气成分。方法以白木香、人工沉香和天然沉香为材料,采用SHS-GC-MS技术不经任何化学处理,直接加热提取香气成分进行分析,并应用自动去卷积系统结合保留指数对香气成分进行鉴定。结果天然沉香、人工沉香和白木香中共检出55种挥发性成分,主要为醛、酮、酚醇、酯、萘、烃、2-(2-苯乙基)色酮等化合物。天然沉香、人工沉香中出现7种共有成分,主要为酮类和酚醇类化合物;天然沉香独有7种成分,分别属于酚醇类、酮类和萘类化合物;人工沉香中酮类化合物含量较高,而天然沉香中含量最高的为酚醇类化合物,两者的香气成分存在明显差别。结论该法体现了人工沉香和天然沉香的香气物质基础,可为沉香品质评价提供有效、可行的方法。
Objective To analyze aroma components of agarwood by static headspace sampling gas chromatography- mass spectrometry（SHS-GC-MS） combined with retention index （RI）. Methods Aroma components of white-wood, artificial agarwood and natural agarwood were heated and extracted directly for analysis by SHS-GC-MS without any chemical process, and the components were identified by using automated mass spectral deconvolution ＆ identification system （AMDIS） combined with retention index. Results There were 55 aroma components identified in this study, like aldehydes, ketones, phenol alcohols, esters,naphthalenes, hydrocarbons, and 2-（2-phenylethyl） chromones. There were seven common components present in the artificial and natural agarwood,mainly belong to ketones,alcohols and phenols.And the 7 components only present in the natural agarwood belong to phenol alcohols, ketones and naphthalenes.Ketones and phenol alcohols possess the highest content in artificial agarwood and natural agarwood,respectively.There were significant differences in aroma components between artificial agarwood and natural agarwood. Conclusion This method is effective and feasible for quality evaluation of agarwood.
作者
钟秋萍
钟兆健
陈晓东
袁宁
詹松
高晓霞
陈晓颖
ZHONG Qiuping ZHONG Zhaojian CHEN Xiaodong YUAN Ning ZHAN Song GAO Xiaoxia CHEN Xiaoyingl(School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China Shimadzu Analytical Applications Center Guangzhou, Shimadzu （China） Co., LTD., Guangzhou 510010, China)
出处
《医药导报》
CAS
2016年第11期1243-1249,共7页
Herald of Medicine
基金
广东省基础与应用基础研究专项资金(2014A030313583)
广东省科技计划项目(2012A030100014)
广东药学院大学生创新创业训练计划项目(1057313018)
广东药学院药科学院青年教师科研培育基金(2014No.5)
关键词
沉香
香气成分
气相色谱-质谱联用
静态顶空进样
Agarwood
Aroma components
Gas chromatography-mass spectrometry, static head space sampling
