摘要
目的观察柚皮苷对醋酸所致胃溃疡大鼠血清及胃黏膜组织中三叶因子2(TFF2)含量的影响,探讨柚皮苷对胃溃疡的治疗作用。方法将45只SD大鼠随机分成正常对照组,模型对照组,柚皮苷大、小剂量组。除正常对照组外,其他组采用改良Okabe法制作醋酸型胃溃疡模型。造模后次日,模型对照组给予0.9%氯化钠溶液1 m L·(100 g)-1灌胃;柚皮苷小剂量组给予柚皮苷50 mg·kg-1灌胃;柚皮苷大剂量组给予柚皮苷100 mg·kg-1灌胃。均每天1次,连续给药1周后测量胃溃疡面积,酶联免疫吸附(ELISA)法检测血清与胃黏膜组织TFF2含量。结果与模型对照组比较,柚皮苷小剂量组与大剂量组溃疡指数均明显降低(P<0.01),大鼠血清及胃黏膜TFF2含量明显升高(P<0.05),且大剂量组明显优于小剂量组((P<0.01)。结论柚皮苷具有显著抗大鼠胃溃疡作用,其作用机制可能与提高血清及胃黏膜组织TFF2含量有关。
Objective To explore the effects of naringin on trefoil factor 2 （TFF2） content in serum and gastric mucosa tissue in gastric ulcer rats induced by acetic acid and its therapeutic effects on gastric ulcer. Methods Forty-five rats were randomly divided into normal control group, model control group and naringin high and low dose groups.The acetic acid gastric ulcer model was established by modified okabe method. On the next day, model control group was given 0.9% sodium chloride solution 1 mL · （ 100 g） -1 ,naringin low-dose group was given 50 mg ·kg-1, and naringin high-dose group was given 100 mg · kg-1, all by gastric perfusion once-daily for 7 days.Gastric ulcer area was measured, and the contents of TFF2 in serum and gastric mucosa tissue were detected by enzyme linked immunosorbent assay （ELISA）. Results Compared with the model control group, the ulcer index in naringin treatment groups were significantly decreased （P〈 0.01 ）, and the contents of TFF2 in serum and gastric mucosa tissue were significantly increased （P〈0.05）. The high dose group was obviously superior to the low dose group （P〈0.01）. Conclusion Naringin has significant anti-gastric ulcer effects and the mechanism may be associated with the increased content of TFF2 in serum and gastric mucosa tissue.
作者
秦建设
郑波
QIN Jianshe ZHENG Bo(Chongqing Three Gorges Medical College, Chongqing 404120, China)
出处
《医药导报》
CAS
2016年第11期1194-1197,共4页
Herald of Medicine
基金
重庆市教育委员会科学技术研究资助项目(KJ131805)
关键词
柚皮苷
溃疡
胃
三叶因子2
Naringin
Ulcer, gastric
Trefoil factor 2
