摘要
目的:探讨七方胃痛颗粒对慢性萎缩性胃炎模型大鼠胃黏膜组织中TFF1、TFF2表达的影响。方法:100只Wistar大鼠按照随机数字表法分为模型对照组、七方胃痛颗粒高剂量组(以下简称高剂量组)、七方胃痛颗粒中剂量组(以下简称中剂量组)、七方胃痛颗粒低剂量组(以下简称低剂量组)、胃复春组,每组20只,五组均采用化学致癌物N-甲基-N-硝基-N-亚硝基胍(MNNG)建立慢性萎缩性胃炎动物模型,以胃复春为对照组,采用免疫组化染色超敏2步法观察七方胃痛颗粒对大鼠慢性萎缩性胃炎TFF1和TFF2的作用。结果:与模型组比较,高剂量组、中剂量组、低剂量组及胃复春组能显著增强TFF1、TFF2的表达(P<0.05);与胃复春组比较,高剂量组TFF1、TFF2表达显著增强(P<0.05)。结论:七方胃痛颗粒防治慢性萎缩性胃炎的作用机制可能是通过促进TFF1、TFF2的表达,从而发挥对黏膜保护作用。
Objective： To explore the effect of Qifang Weitong Granules on expression of gastric mucosa TFF1,TFF2 of patients with chronic atrophic gastritis.Method： 100 Wistar rats were randomly divided into model group,high dose group,medium dose group and low dose group of Qifang Weitong Granules,Weifuchun group,20 rats in each group.The five groups were treated with chemical carcinogens N-methyl-N,nitro-N-nitrosoguanidine（MNNG） to establish animal model with chronic atrophic gastritis.Weifuchun group as control group,the immunohistochemical staining of two-step hypersensitivity was used to observe the effect of Qifang Weitong Granules on TFF1 and TFF2 of rats with chronic atrophic gastritis.Results： Compared with the model group,high dose group,middle dose group,low dose group and Weifuchun group can significantly enhance TFF1and TFF2 expression（P0.05）;compared with Weifuchun group,TFF1 and TFF2 expression of high dose group was significantly increased（P0.05）.Conclusion： Qifang Weitong Granules can treat chronic atrophic gastritis and its role of the mechanism may be through the promotion of TFF1 and TFF2 expression,thus playing a protective effect on the mucosa.
出处
《河南中医》
2013年第1期51-53,共3页
Henan Traditional Chinese Medicine
基金
广西研究生教育创新计划资助项目(编号:2011106001005M19)
