摘要
探讨灵芝(Ganoderma lucidum)孢子油提取物复合制剂对小鼠急性酒精肝损伤的保护作用。分别设定0.167 g·kg^(-1)、0.333 g·kg^(-1)、1.00 g·kg^(-1)三个剂量组,以及乙醇模型对照组和空白对照组。各剂量组经口灌胃给予受试物,溶剂对照组和乙醇模型组给予食用植物油。30 d后,用乙醇建立急性酒精性肝损伤小鼠模型。16 h后处死动物,测定肝组织中甘油三酯(TG)、谷胱甘肽(GSH)、丙二醛(MDA),同时取肝脏进行组织病理学检查。结果表明样品可以降低3个剂量组小鼠急性酒精性肝损伤后第16小时肝组织中TG含量和中剂量组MDA含量,肝脏组织病理学检查发现,乙醇模型对照组动物肝损伤程度高于溶剂对照组(P<0.05)。结论证明灵芝孢子油提取物复合制剂对急性酒精肝损伤具有辅助保护功能。
Effects of Ganoderma lucidum spore oil and extract complex preparation on mice with alcoholic liver injury were studied in this paper. The experiment was employed with 5 treatments, such as 0.167 g·kg^-1, 0.333 g·kg^-1, 1.00 g·kg^-1 dosage group, blank control and ethanol model control group. Three dosages of samples were given orally with blank control and ethanol model control groups given edible vegetable oil. 30 days later, the mouse model of acute alcoholic liver injury was established by ethanol. The animals were sacrificed after 16 hours, and the livers were collected for the triglycercide （TG）, glutathione （GSH）, methane dicarboxylic aldehyde （MDA） measurements and liver histopathological examination. The results showed that three dosages of samples significantly reduced the TG content in mice liver tissue at the 16th hour after the alcoholic treatments while the middle dosage sample reduced the MDA content. And the ethanol model control group showed higher damage degree than the blank control group （P〈0.05）. It was concluded that G. lucidum spore oil and extract complex preparation exhibited a protective effect on acute alcohol liver injury.
出处
《中国食用菌》
2016年第6期34-37,共4页
Edible Fungi of China
基金
公益性行业(农业)科研专项的部分研究内容(201303080)
关键词
灵芝孢子油提取物复合制剂
酒精性肝损伤
乙醇模型
ICR小鼠
Ganoderma lucidum spore oil and extract complex preparation
alcoholic liver injury
ethanol model
ICR mice
