摘要
目的:采用星点设计-效应面法优化太白楤木总皂苷磷脂复合物(TSAT-PC)的制备工艺,并对其理化性质进行研究。方法:采用溶剂挥发法制备TSAT-PC,单因素试验考察反应溶剂、反应时间、反应温度、投料比、药物浓度对复合率的影响,星点设计-效应面法优化制备工艺,并对形成的磷脂复合物进行理化性质研究。结果:最佳制备工艺条件:反应溶剂反应时间1 h、反应温度45℃、大豆磷脂(SL)与TSAT的投料比3∶1、药物浓度16 mg/m L,复合率可达97.23%,与预测值偏差<5%;IR验证了磷脂复合物的形成,其在正辛醇中的溶解度有了较大提高。结论:采用星点设计-效应面法成功制备了TSAT-PC,改善了脂溶性,为中药制剂的进一步开发利用提供了参考。
Objective： To optimize the preparation of total saponins of Aralia taibaiensis phospholipid complex（ TSAT-PC） by the central composite design-response surface method. Methods： Total saponins of Aralia taibaiensis phospholipid complex was prepared by using solvent evaporation method,five factors including reaction solvent,reaction time,reaction temperature,ratio of reactants on this reaction,and the concentration of the drug were investigated,then to optimize the preparation of TSAT-PC by the central composite designresponse surface method,and to study its physicochemical properties. Results： The optimal process conditions were as follows,the reaction time was 1 h,the reaction temperature was 45 ℃,the ratio of soya lecithine（ SL） and TSAT was 3∶ 1,the reaction concentration was16 mg / m L,the complexing rate was 97. 23%,it was less than 5% with the predicted deviation; IR analysis proved the formation of TSAT-PC,and the solubility in the octyl alcohol was higher than the original drug. Conclusion： TSAT-PC was successfully developed by the optimized process,enhance the solubility in octyl alcohol,which provide the reference for the further development and utilization of Chinese materia medica preparation.
作者
翟秉涛
李佳
柳小莉
杨伟平
郑蓓
史亚军
郭东艳
ZHAI Bing-tao LI Jia LIU Xiao-li YANG Wei-ping ZHENG Bei SHI Ya-jun GUO Dong-yan(The Medicine College of Shaanxi University of Chinese Medicine, Xianyang 712046, China)
出处
《中药材》
CAS
CSCD
北大核心
2016年第6期1361-1364,共4页
Journal of Chinese Medicinal Materials
基金
陕西省科技统筹项目(2013KTCQ03-12)
陕西省科技计划项目(2014JM4104)
陕西中医药大学校基金(2015PY13)
关键词
太白楤木总皂苷
磷脂复合物
星点设计-效应面法
Total saponins of Aralia taibaiensis
Phospholipid complex
Central composite design-response surface method
