摘要
以优良植物生防菌解淀粉芽孢杆菌L-S60为研究对象,通过单因素试验研究酸碱、盐浓度、温度对该菌株生长的影响;检测解淀粉芽孢杆菌L-S60的产酶和抑菌特性;并研究优化解淀粉芽孢杆菌L-S60的固态发酵工艺。结果表明:解淀粉芽孢杆菌L-S60具有较宽的酸碱耐受性,盐的质量分数>10%时菌落数会显著下降,80℃以下菌落数无明显变化。解淀粉芽孢杆菌L-S60能产生蛋白酶、纤维素酶和淀粉酶,且对多种病原真菌有抑制作用。L-S60的最佳固体发酵条件为:温度35℃、接种量30%、料水质量比1∶0.45、装瓶量40%和发酵时间42h;最佳固体培养基组成为,m(花生饼粉)∶m(麦麸)∶m(棉粕)=53∶35∶12,外源物质蛋白胨、葡萄糖和MgSO4·7H2O添加量分别为3.00、2.00和0.20g/kg,此时,解淀粉芽孢杆菌L-S60菌落数达到2.42×1010 CFU/g。解淀粉芽孢杆菌L-S60具有很好的开发利用价值。
The impacts of concentration of acid, alkali, salt and the temperature on the growth of Bacillus amyloliquefaciens L-S60 were investigated through single factor experiments. The biological features of L-S60 were studied and the solid-state fermentation condition of this strain was optimized in this study. The results showed that..The resistance of B. amyloliquefaciens L-S60 to acid and alkali is robust; the colony number of L-S60 dropped significantly when the salt concentration exceeded 10%; there was no significant difference of the colony number of L-S60 when the temperature is under 80℃. Strain L-S60 could produce amylase, protease and cellulose. It also showed strong inhibitory effects on a variety of pathogenic fungi. In order to make B. amyloliquefaciens L-S60 a good bio-fertilizer, the solid fermentation conditions and medium composition were also explored. The optimum temperature was 35℃, the inoculum concentration was 30% and the ratio of material and water was 1 ： 0.45. We chose 40% as the amount of bottling and 42 h as the fermentation time. The best solid fermentation medium contained 53% of peanut shell powder,35% of wheat bran,and 12% of cottonseed meal. Finally, extra nitrogen source （3. 00 g/kg）, carbon source （2. 00 g/kg）, and inorganic salt （MgSO4.7H2O 0.20 g/kg） were added in the solid state fermentation medium. Under this condition, the number of B. amyloliquefaciens colonies L-S60 reached 2.42×10^10CFU/g.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2016年第9期133-142,共10页
Journal of China Agricultural University
基金
公益性行业(农业)科研专项经费项目
关键词
解淀粉芽孢杆菌
生物学特性
固态发酵
菌落数
Bacillus amyloliquefaciens
biological characteristics
solid-state fermentation
the number of colonies
