摘要
以脂多糖(LPS)致大鼠氧化损伤为模型研究微生物源性抗氧化剂(microbe-derived antioxidantm,MA)对大鼠抗氧化功能和肝脏损伤修复的作用。选用100只雄性SD大鼠(185.74±6.86)g,随机分成4组,分别为对照组、LPS组、LPS+0.5MA组和LPS+1.0MA组。各处理组试验鼠均饲喂基础日粮,LPS+0.5MA组和LPS+1.0MA组饮水中分别添加MA 0.5mL/只/d和1.0mL/只/d;试验第29天,LPS组、LPS+0.5MA组和LPS+1.0MA组分别腹腔注射LPS 3mg/kg,对照组注射等剂量的生理盐水。结果表明,MA可显著提高正常大鼠血清和肝脏中超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05);MA处理后,显著减缓LPS所致的血清和肝脏SOD和GSH-Px酶活性下降(P<0.05),显著降低血清和肝脏丙二醛(MDA)和一氧化氮(NO)水平(P<0.05),有降低血清谷草转氨酶(AST)和谷丙转氨酶(ALT)活性的趋势(P>0.05)。结论,MA具有提高大鼠抗氧化能力,修复肝脏氧化损伤的作用,且呈一定的剂量效应。
To investigate the protective effects of microbe-derived antioxidant（MA）on antioxidant ability and LPS-induced injury in rats,100 male SD rats with similar body weight were randomly divided into four groups including the control,LSP,LSP ＋ 0.5 MA and LSP ＋ 1.0 MA.All rats were fed basal diets and added 0.5 mL/n/d MA and 1.0 mL/n/d MA in LSP ＋ 0.5 MA group and LSP ＋ 1.0 MA group,respectively;On the 29 thday of the trial,the rats of damage groups（LSP,LSP ＋0.5MA,LSP ＋1.0MA）were intraperitoneally injected 3 mg/kg LSP and control group received equal amounts of normal saline.MA can significantly increase the activities of SOD and GSH-Px in serum and liver（P〈0.05）and slow down the decrease of them（P〈0.05）.Futhermore,MA can also significantly decreased the contents ofMDA,NO（P〈0.05）both in serum and liver tissue,reduced the contents of AST and ALT（P〉0.05）in serum.MA can increase antioxidant ability of rats and repair the liver oxidative damage in a dose-dependent manner.
出处
《上海交通大学学报（农业科学版）》
2016年第5期17-22,共6页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
公益性行业(农业)科研专项(201303143-06-01)
