摘要
本文旨在研究植物乳杆菌对单增李斯特菌的抑制作用,并建立植物乳杆菌对单增李斯特菌的抑制模型。通过稀释法获得植物乳杆菌纯菌培养液及植物乳杆菌/单增李斯特菌的混合培养液,分别置于7℃、14℃、21℃、28℃与35℃恒温培养箱中培养并适时取样计数,单增李斯特菌纯培养数据从Com Base数据库获得。基于两种菌的混合培养数据,应用Baranyi模型为初级模型,拟合得到两种菌的最大比生长速率和迟滞期,进而应用改良的Jameson-effect模型建立植物乳杆菌对单增李斯特菌的抑制模型,并对模型进行数学检验。结果显示,7℃时植物乳杆菌不生长;14℃、21℃、28℃与35℃条件下,植物乳杆菌对单增李斯特菌有显著抑制作用。抑制模型数学检验结果为均方误差值在0.07-0.10之间,偏差因子值在0.98-1.04之间,准确因子值在1附近,表明所建立改良的Jameson-effect模型能够较好地预测植物乳杆菌作为抑菌剂存在条件下单增李斯特菌的生长情况。
The inhibitory effect of Lactobacillus plantarum on the growth of Listeria monocytogenes was investigated, and a model for demonstrating this inhibition was constructed using a modified Jameson-effect model. A Lac. plantarum pure culture and a mixed culture of Lac. plantarum and Lis. monocytogenes were obtained using the dilution method, and were cultivated in thermostat incubators at 7 ℃, 14 ℃, 21 ℃, 28 ℃ and 35 ℃, respectively. Samples were collected periodically and the bacterial count was recorded. The Lis. monocytogenes pure culture data were obtained from the Com Base database. Based on the data of the mixed culture, the Baranyi model was selected as the primary model and was used for fitting to estimate the maximum growth rate（μmax） and lag phase（λ）. Subsequently, the modified Jameson-effect model was employed to establish the model for the inhibition of Lis. monocytogenes by Lac. plantarum, and validation of the model was performed. Lac. plantarum did not grow at 7 ℃ and exhibited a significant inhibitory effect on Lis. monocytogenes at 14 ℃, 21 ℃, 28 ℃ and 35 ℃. Validation of the inhibition model showed that the mean square error（MSE）, bias factor（Bf）, and accuracy factor（Af） values were in a range of 0.07-0.10, 0.98-1.04 and -1, respectively, indicating that the modified Jameson-effect model could effectively predict the growth of Lis. monocytogenes in the presence of Lac. plantarum as an inhibitor.
出处
《现代食品科技》
EI
CAS
北大核心
2016年第9期135-140,共6页
Modern Food Science and Technology
基金
科技部"十二五"国家科技支撑计划课题项目(2015BAK36B04)
国家自然科学基金面上项目(31271896
31371776)
农业部农产品质量安全风险评估实验室(杭州)开放基金课题(2015FXPG01)
上海市科委2015年长三角科技联合攻关领域项目(15395810900)
