摘要
目的通过蓝莓联合益生菌减少CXCL10/CXCR3的表达对非酒精性脂肪肝(NAFLD)的影响及作用机制。方法清洁级SD大鼠30只分为正常组(normal group,N组)、模型组(model group,M组)、蓝莓组(blueberry group,B组)、益生菌组(probiotics group,P组)和蓝莓益生菌组(blueberry+probiotics group,BP组)。除N组(100%普通饮食)外,其余大鼠均采用复合高脂饲料(88.2%普通饲料+10%猪油+1.5%胆固醇+0.2%胆酸钠)制备NAFLD模型共12周。确认造模成功后再将剩余M组大鼠随机分为B组(蓝莓原浆1.5 m L/(100 g·d)灌胃)、P组(益生菌溶液1.5 m L/d灌胃)及BP组(蓝莓益生菌原液1.5 m L/100 g体重灌胃),并给普通饮食,共观察8周。结果 BP组与M组、B组、P组比较:肝脏指数、血清丙氨酸氨基转移酶、门冬氨酸氨基转移酶、总胆固醇、三酰甘油均差异有统计学意义(P<0.01)。免疫组化:BP组的CXCL10/CXCR3表达较M组、B组、P组减少(P<0.01)。RT-PCR:BP组的CXCL10/CXCR3表达与M组、B组、P组比较显著降低(P<0.01)。Western blot:BP组的CXCL10/CXCR3表达较M组、B组、P组显著降低(P<0.01)。结论蓝莓联合益生菌对比单独使用蓝莓或益生菌能有效改善NAFLD的病理组织结构,减轻肝细胞脂肪变性,其机制可能是蓝莓与益生菌联合可减轻CXCL-10诱发的炎症反应,下调三酰甘油相关基因的表达,减少脂质沉积,是改善NAFLD的一个辅助治疗方案。
Objective To observe the effects of probiotics in combination with blueberry on the expression of CX- CL10/CXCR3 in rats with non - alcoholic fatty liver disease （NAFLD） and to investigate the potential mechanisms. Methods Thirty clean grade rats were randomly divided into the normal, model, blueberry, probiotics and blueberry plus probiotics groups. The subjects in normal group （n =6） were fed with 100% standard diet. The other rats were in- duced by complex high fat diet for 12 weeks and treated with blueberry juice, probiotics solution and blueberry juice plus probiotics solution, respectively, for 8 weeks. Results The liver indexes, ALT, AST, TG, TC were significantly lower in Group BPG than those in Group MG, BG and PG （P 〈 0. 01 ）. Immunohistochemistry staining indicated that the positive expression of CXCL - 10 and CXCR -3 in Group BPG was significantly lower than those in Group MG, BG and PG （P 〈 0. 01 ）. According to RT - PCR, the mRNA expression of CXCL - 10 and CXCR - 3 in Group BPG was significantly down - regulated when comparing to those in Group MG, BG and PG （P 〈0. 01 ）. According to Western blot, the expression of CXCL - 10 and CXCR - 3 was also significantly declined in Group BPG （P 〈 0. 01 ）. Conclusion Blueberry in combina- tion with probiotics could effectively ameliorate pathological changes in hepatic tissues in NAFLD and attenuate steatosis of hepatocyte, via reduction in inflammatory factor reaction, meanwhile amplifying cholesterol metabolism and attenuating lip- id deposit in liver.
出处
《广东医学》
CAS
北大核心
2016年第19期2845-2850,共6页
Guangdong Medical Journal
基金
贵州省科技厅社会攻关资助项目(编号:黔科合SY[2010]3017号)
黔东南苗族侗族自治州林业局科研基金资助项目(编号:ZLYJZFCG-2012-7)
