摘要
背景:慢性阻塞性肺疾病是一种具有气流受限特征的以炎症为核心的慢性呼吸系统疾病。目的:探讨脂肪间充质干细胞对慢性阻塞性肺疾病大鼠的治疗作用。方法:健康雄性SD大鼠60只随机分成3组(n=20),治疗组和模型组大鼠通过烟熏及气管内注入脂多糖的方法建立慢性阻塞性肺疾病模型,对照组大鼠不进行任何处理。造模后治疗组大鼠经尾静脉注射CM-Dil标记的脂肪间充质干细胞,模型组及对照组给予等量生理盐水,移植后14 d,检测大鼠肺通气功能、炎症因子水平和肺组织病理学变化。结果与结论:(1)与对照组比较,模型组和治疗组肺功能明显下降,治疗组肺功能较模型组明显改善(P<0.05);(2)治疗组肺泡灌洗液中白细胞总数、巨噬细胞数及中性粒细胞数均显著低于模型组(P<0.05);(3)治疗组肺泡灌洗液中白细胞介素8、肿瘤坏死因子α、C-反应蛋白水平低于模型组(P<0.05);(4)与模型组相比,治疗组血清中超氧化物歧化酶活性显著增高、丙二醛水平显著降低(P<0.05);(5)治疗组较模型组肺气肿样改变减轻;(6)CM-Dil标记的脂肪间充质干细胞能够归巢到慢性阻塞性肺疾病大鼠肺组织并存活;(7)结果表明,脂肪间充质干细胞移植能改善慢性阻塞性肺疾病大鼠肺功能,减轻炎症反应,具有一定治疗作用。
BACKGROUND： Chronic obstructive pulmonary disease is progressive respiratory disease characterized by airflow limitation.OBJECTIVE： To investigate the therapeutic effect of adipose-derived mesenchymal stem cell transplantation in rats with chronic obstructive pulmonary disease. METHODS： Sixty healthy male Sprague-Dawley rats were randomly divided into control, model and treatment groups（n=20 per group）. Rat models of chronic obstructive pulmonary disease were made in the model and treatment group, while no treatment was done in the control group. After modeling, rats in the treatment group were given tail vein injection of CM-Dil-labeled adipose-derived mesenchymal stem cells, and rats in the model and control groups given the same amount of normal saline. Rat pulmonary ventilation function, inflammatory factor level and pathological changes of the lung were detected at 14 days after cell transplantation. RESULTS AND CONCLUSION： After modeling, reduced lung function was found in rats with chronic obstructive pulmonary disease, but the cell transplantation significantly improved this reduction（P〈0.05）. Compared with the model group, significantly reduced indexes were visible in the treatment group（P〈0.05）, including the total number of white blood cells, the number of macrophages and neutrophils, levels of interleukin-8, tumor necrosis factor α and C-reactive protein in the bronchoalveolar lavage fluid as well as serum level of malondialdehyde, while serum superoxide dismutase activity was increased significantly in the treatment group（P〈0.05）. In the treatment group, emphysema-like changes were mitigated and CM-Dil-labeled adipose-derived mesenchymal stem cells were capable of homing to the lung tissue of rats with chronic obstructive pulmonary disease and survived. All these findings show that adipose-derived mesenchymal stem cell transplantation can improve lung function of rats with chronic obstructive pulmonary disease, and reduce inflammatory response, which has for certain som
出处
《中国组织工程研究》
CAS
北大核心
2016年第41期6177-6182,共6页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
移植
慢性阻塞性肺疾病
脂肪间充质干细胞
炎症因子
细胞移植
,Pulmonary Disease,Chronic Obstructive
Adipose Tissue
Mesenchymal Stem Cell Transplantation
Tissue Engineering
