摘要
目的观察软骨脱细胞基质(Cartilage acellular extracellular matrix,CAEM)-Ⅱ型胶原(CollagenⅡ,COLⅡ)纳米支架,复合骨髓基质干细胞(Bone marrow stem cells,BMSCs)修复兔关节软骨缺损的效果。方法 CAEM和COLⅡ按质量比1∶1混合,通过静电纺丝技术制备组织工程纳米支架。将第二代BMSCs种植到该支架上,培养箱内静置2 h。12只日本大耳白兔随机分为实验组和对照组,将细胞支架复合物植入实验组兔膝关节软骨缺损处,对照组仅行膝关节软骨缺损建模。12周后实验动物取材,大体观察修复效果,并行HE染色、Ⅱ型胶原染色观察。结果大体观察见实验组软骨缺损修复良好,对照组软骨缺损处由肉芽样组织充填。HE染色显示,实验组关节软骨缺损处可见软骨陷窝形成,对照组关节软骨缺损处仅有纤维组织充填。实验组修复区Ⅱ型胶原染色为阳性,对照组为阴性。结论 CAEM-COLⅡ纳米支架复合BMSC,对兔关节软骨缺损具有较好的修复能力,具有潜在的临床应用价值。
Objective To observe the effect of CAEM-COL Ⅱ nanofiber scaffolds composite with BMSCs in repairing articular cartilage defect of rabbits. Methods Cartilage acellular extracellular matrix （CAEM） and collagen Ⅱ （COL Ⅱ） were mixed according to the ratio of 1：1, nanofiber scaffolds were prepared by electrostatic spinning for cartilage tissue engineering. The second generations of bone marrow stem cells （BMSCs） were inoculated onto the scaffolds. The cell scaffold composite was placed in the incubator for two hours. 12 Japanese white rabbits were randomly divided into the experimental group and the control group. In the experimental group, the cell scaffold composite was implanted into the articular cartilage defects of rabbits. In the control group, only the knee joint cartilage defects were made. After 12 weeks, the animals were sacrificed and the repair effect was observed by gross appearance, HE staining and collagen type lI staining. Results General shape observation showed that cartilage defects in the experimental group were repaired well, and were filled with granulation tissue in the control group. HE staining showed that cartilage cavities formed in articular cartilage defects in the experimental group, and fibrous tissues were filled in the control group. In the repair area, collagen type Ⅱ staining was positive in the experimental group, and negative in the control group. Conclusion The CAEM-COL Ⅱ nanofiber scaffolds composite with BMSCs could well repair the articular cartilage defects of rabbits, and have potential of application in tissue engineering cartilage.
出处
《组织工程与重建外科杂志》
2016年第5期281-284,共4页
Journal of Tissue Engineering and Reconstructive Surgery
基金
国家自然科学基金青年基金(81301568)
川北医学院博士科研启动基金(CBY15-QD03)
关键词
组织工程
Ⅱ型胶原
软骨脱细胞基质
软骨缺损
骨髓基质干细胞
Tissue engineering
Collagen Ⅱ
Mixing cartilage acellular extracellular matrix
Cartilage defect
Bone marrow stem cells
