摘要
目的：探讨检测视黄醇结合蛋白和膜联蛋白 A2水平对糖尿病患者早期肾损伤诊断的意义。方法选取80例糖尿病肾病患者，以24-UAER含量为标准由低到高将患者分为 A组（25例）、B组（26例）和 C组（29例），另选体检者25例作为对照组（NC），测定所有研究对象糖化血红蛋白（HbAlc）、血肌酐（Scr）、尿素氮（BUN）、尿酸（UA）和视黄醇结合蛋白（RBP）水平。实时荧光定量PCR检测膜联蛋白 A2（AnnexinA2）表达水平。结果①病例组（A＋B＋C）的 HbAlc，Scr， BUN，UA和 RBP水平均高于对照组（t＝4.64～13.65，均P＜0.05）；A组和 B组 HbAlc，Scr，BUN和 UA水平比较差异无统计学意义（t＝0.31～1.25，均P＞0.05），而 RBP 水平差异有统计学意义（t＝15.26，P＜0.05）。A组和 C组 Scr， BUN，UA和 RBP水平比较差异有统计学意义（t＝5.26～25.33，均P＜0.05）。B组和C组 Scr，BUN，UA和 RBP水平比较差异也有统计学意义（t＝4.02～18.33，均P＜0.05）；②病例组（A＋B＋C组）AnnexinA2表达水平1.58±0.05 ng／ml，对照组为0.13±0.01 ng／ml，两组比较差异有统计学意义（t＝18.45，P＜0.05）。病例组 A组0.12±0.01 ng／ml，B组1.35±0.06 ng／ml，C组1.88±0.07 ng／ml，两两比较差异有统计学意义（t＝13.45～24.25，均P＜0.05）。③糖尿病肾病组（A＋B＋C）患者 RBP水平与 AnnexinA2表达程度之间存在正相关（r＝0.95，P＜0.01）。结论联合检测血清视黄醇结合蛋白和膜联蛋白 A2是反映糖尿病患者早期肾病微血管病变的敏感指标。
Objective To explore the significance of detection serum Retinol-Binding Protein and AnnexinA2 in diabetic ne-phropathy.Methods According to 24-UAER level,80 diabetic patients with suspected DN were devided into three groups, that was A （25 cases）,B （26 cases）and C （29 cases）group,and control group （NC）was 25 cases.HbA1c,BUN,Scr,UA and RBP leves were detected by the standard method.The expression level of Annexin A2 were detected by real-time PCR. Results There were obvious differences of HbA1c,BUN,Scr,UA and RBP levels among three groups and NC group （t=4.64~13.65,P〈0.05）,To HbA1c,BUN,Scr and UA,no differences between A group and B groups （t=0.31~1.25,P〉0.05）,there were differences of RBP （t=15.26,P〈0.05）.To HbA1c,BUN,Scr UA and RBP,there were differences be-tween A groups and C groups （t=5.26~25.33,P〈0.05）,there were the same results between B groups and C groups （t=4.02~18.33,P〈0.05）.To expression level of AnnexinA2 there were differences among three group and NC group （（t=13.45~24.25,P〈0.05））.There exist positive correlation between the expression of AnnexinA2 and RBP level （r=0.95, P〈0.01）.Conclusion Combined detection of serum RBP and expression level of AnnexinA2 may be a sensitive and early phase indicator for impairment of renal function for diagnosis of diabetic nephropathy.
出处
《现代检验医学杂志》
CAS
2016年第5期118-119,122,共3页
Journal of Modern Laboratory Medicine
