摘要
B3超家族基因是植物中特有的一类转录因子,参与植物生长发育、形态建成及抗逆境胁迫等过程。从玉米黄早4中获得一个含有2个B3-DNA结合域的基因,为B3超家族基因,命名为ZmREM。该基因全长1 047 bp,编码含有349个氨基酸的蛋白。Blast比对结果表明,ZmREM和谷子含有B3结构域蛋白、高粱的假定蛋白相似性分别为81%和84%。ZmREM基因是组成型表达,在根中表达量最高。同时ZmREM基因的转录水平可以为干旱、盐、冷和热所诱导。因此,ZmREM可能参与玉米多种非生物胁迫应答途径。
The plant-specific B3 superfamily constituted a large transcription factor superfamily. They play central roles in plant development,morphogenesis and stress-resistance. Taking total RNA of maize as template,REM gene was cloned by use the method of reverse transcription polymerase chain reaction( RT-PCR). Length of 1 047 bp c DNA sequence that encoding 349 amino acids was obtained. CD search result showed that it contained two B3-DNA domains and was named as ZmREM. Blast result indicated that ZmREM had 84% and 81% homology with Sorghum bicolor hypothetical protein and Setaria italica B3 domain-containing protein. In various tissues,gene expression in root was the highest. The expression of ZmREM in maize was induced by heat,high-salt,cold and PEG treatment.These results suggested that ZmREM might be a stress related gene of maize.
出处
《浙江农业学报》
CSCD
北大核心
2016年第11期1822-1827,共6页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(31101155)
河北北方学院重大项目(ZD201407,ZD201305)
河北省科技厅项目(13226326)
关键词
玉米
REM转录因子
基因克隆
非生物胁迫
maize(Zea mays L.)
REM transcription factor
gene cloning
abiotic stress
