摘要
药用植物作为中药和世界传统药物的主要来源,面临着资源稀缺和活性成分含量低等问题.通过转录水平调控发育相关基因及活性成分合成途径酶基因表达是实现定向、高效调节药用植物生长及活性成分合成的有效手段之一.因此,近年来转录因子调节药用植物发育及活性成分合成的研究备受关注.转录因子AP2/ERF家族是植物最大转录因子家族之一,家族成员均包含保守AP2结构域,根据结构域数量和识别序列不同,AP2/ERF家族被分为5个亚家族:AP2(APETALA2),ERF(ethylene-responsive factor),DREB(dehydration-responsive element binding proteins),RAV(related to ABI3/VP1)和Soloist.本文重点综述转录因子AP2/ERF调控药用植物活性成分生物合成、发育、胁迫响应的研究进展,阐述了转录因子AP2/ERF调控靶基因和自身受到调控的作用机制,同时总结转录因子AP2/ERF研究方法,提出组学和生物信息学方法成为分离、筛选转录因子,预测转录因子功能的强大工具,为分析、预测、验证药用植物AP2/ERF家族成员的功能和阐明AP2/ERF的调控机制提供理论基础和方法指导.转录因子AP2/ERF的功能研究及其作用机制的揭示将有助于利用代谢调控手段提高药用植物活性成分产量,有利于药用植物优良品种的培育,为满足人们对天然药物的需求奠定基础.
As a major source of Chinese medicines and traditional drugs worldwide, medicinal plants are facing challenges such as resource scarcity and low bioactive compound content. Regulating gene expression by transcription factors （TFs） is an effective method to coordinate the development of medicinal plants and the biosynthesis of active compounds. Therefore, many researches have been focused on TFs. As one of the largest transcription factor families, AP2/ERF TFs contain at least one AP2 DNA binding domain. This gene family is divided into five subfamilies, namely AP2 （APETALA2）, ERF （ethylene-responsive factor）, DREB （dehydration-responsive element binding proteins）, RAV （related to ABI3/VP1） and Soloist. This review emphasizes that AP2/ERF TFs regulate the biosynthesis of active compounds, development and stress responses of medicinal plants. The regulatory mechanism and research methods for AP2/ERF TFs are also elaborated. Genomics, transcriptomics, and bioinformatics are proposed to be powerful tools for isolation, screening and prediction of AP2/ERF TFs. This review may serve as a guide for future studies on unknown AP2/ERF TFs. In the future, knowledge of the functions and regulatory mechanisms of AP2/ERF TFs may contribute to the enhancement of bioactive compound production by metabolic engineering and the breeding of fine varieties of medicinal plants. Such work would help to address the growing global demand for natural medicines.
出处
《科学通报》
EI
CAS
CSCD
北大核心
2015年第14期1272-1284,共13页
Chinese Science Bulletin
基金
国家科技支撑计划(2012BAI29B01)
国家自然科学基金(81373916)资助
关键词
转录因子
AP2/ERF
药用植物
活性成分
生长发育
transcription factors （TFs）, AP2/ERF, medicinal plants, bioactive compounds, growth and development
