摘要
[Objective] This study was conducted to develop a system for simultaneous determination of imidacloprid, diflubenzuron, thiabendazole and carbendazim in fruit juice by HPLC. [Method] Using acetonitrile as the extraction solvent, the pesticides in fruit juice were purified through a NH2 solid phase extraction （SPE） cartridge, then detected by HPLC. [Result] There was a good linear relationship between the peak area and the concentrations of imidacloprid, diflubenzuron, thiabendazole and carbendazim in a range of 0.05-5.0 μg/ml, and the linear correlation coefficient varied in a range of 0.999 0-0.999 8; the limit of detection for imidacloprid, diflubenzuron, thiabendazole and carbendazim was 0.003, 0.005, 0.003 and 0.007 mg/kg, respectively. The recovery rate of imidacloprid, diflubenzuron, thiabendazole and carbendazim standards added at three levels （0.1, 0.5 and 1.0 mg/kg） ranged from 82% to 107%, with RSD less than 4.5%. [Conclusion] The sensitivity, accuracy and precision of this method were able to meet the requirements for pesticide residue analysis.
[目的]建立一种可以同时测定果汁中吡虫啉、除虫脲、噻菌灵和多菌灵农药残留的液相色谱法。[方法]以乙腈作为提取溶剂，采用氨基柱净化、高效液相色谱二极管检测器测定，外标法定量。[结果]4种农药在0．02～5．0μg／ml的浓度范围内呈良好的线性关系，线性相关系数为0．9990—0．9998．检出限分别为0．003、0．005、0．003和0．007mg/kg。在3个添加水平（0．1、0．5和1．0mg／kg）的回收率试验中，吡虫啉、除虫脲、噻菌灵和多菌灵的平均回收率为82％～107％，RSD小于4．5％。[结论]该方法简单、快速，且方法的灵敏度、准确度和精密度均能满足农药残留分析的要求。
基金
Supported by the Special Funds for Supervision on the Quality and Safety of Agricultural Products(GJFP201601503)~~
