摘要
选用巨大芽孢杆菌(Bacillus megatherium)作为实验菌种,以餐厨垃圾湿热处理脱出液为发酵培养基制作解磷液态菌肥。将巨大芽孢杆菌接种于餐厨垃圾湿热处理脱出液中进行培养并确定最佳发酵时间,考察初始p H值、接种量、培养温度、摇床转速、装液量和脱出液与水的混合比例对活菌数的影响。结果表明:巨大芽孢杆菌在餐厨垃圾湿热处理脱出液中最佳发酵时间为36 h,发酵最佳初始p H值、接种量、培养温度、摇床转速、装液量和脱出液与水的混合比例分别为7.5、2%、30℃、210 r/min、50 m L(250 m L锥形瓶)和1∶1。巨大芽孢杆菌在餐厨垃圾湿热处理脱出液中进行培养后可达到农业部规定的液态菌肥的活菌数(2×108cfu/m L)标准。
In this study,Bacillus megatherium was used as the experimental strain to produce phosphorus liquid biofertilizer using effluent of hydrothermal treated restaurant garbage（ EHTRG） as the fermentation medium. Bacillus megatherium was inoculated in EHTRG to determine the optimal incubation time,initial p H value,inoculation amount,culture temperature,shaking speed,medium volume and the mix proportion of EHTRG and water. The results showed that the optimal incubation time was 36 h,the optimum initial p H value,inoculation amount,culture temperature,shaking speed,medium volume and the mix proportion of EHTRG and water were 7. 5,2%,30 ℃,210 r/min,50 m L（ in 250 m L erlenmeyer flask） and 1 ∶ 1,respectively. The number of living Bacillus megatherium cultivated in EHTRG could reach the standard number level of living bacterium in liquid bacterial manure set by the Ministry of Agriculture.
出处
《环境工程》
CAS
CSCD
北大核心
2016年第10期44-48,97,共6页
Environmental Engineering
基金
国家自然科学基金面上项目(51578008)
北京市自然科学基金(8144041)
"十二五"国家科技支撑计划(2014BAC27B01-03)
北京工商大学两科基金培育项目(LKJJ2016-13)
"十二五"国家科技支撑项目(2012BAC25B01)
关键词
巨大芽孢杆菌
餐厨垃圾
湿热处理
液态菌肥
生长条件
Bacillus megatherium
restaurant garbage
hydrothermal treatment
liquid biofertilizer
growth condition
