摘要
目的:测定浓缩生长因子(concentrated growth factors,CGF)凝胶层与红细胞(red blood cells,RBC)层中转化生长因子-β1(transforming growth factor-β1,TGF-β1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)分布及含量,探究TGF-β1、VEGF在CGF凝胶层内的分布及含量与其促成骨效应的相关性并指导临床应用。方法:招募6名健康志愿者,采集静脉血制备CGF样品。免疫组织化学法观察CGF凝胶层与RBC层中TGF-β1、VEGF的分布并测定制备即刻的累积光密度值(integrated optical density,IOD)和平均光密度值(average optical density,AOD)。酶联免疫吸附(enzyme-linked immunosorbent assays,ELISA)测定制备即刻上清层与制备1 d后CGF凝胶层析出液中TGF-β1、VEGF的浓度。结果:TGF-β1与VEGF大量聚集于CGF凝胶层中,在RBC层中仅位于多核细胞胞浆和散在的血小板内;CGF凝胶层与RBC层的交界区域有大量血小板与白细胞聚集,并有较高的TGF-β1表达;CGF凝胶层析出液中TGF-β1和VEGF的浓度分别为(55 236.78±3 686.34)ng/L和(610.99±148.81)ng/L,均显著高于上清层(20 710.20±4 523.14)ng/L和(335.20±51.69)ng/L(P<0.001)。结论:CGF凝胶层内含有大量TGF-β1、VEGF,可发挥促进成骨与加速组织愈合的作用。在临床应用中建议应用时间宜早为佳,除整个CGF层外,可将上清层、CGF层与RBC层交界区域一并使用。
Objective： To investigate the distribution and content of transforming growth factor-β1（ TGF-β1） and vascular endothelial growth factor（ VEGF） in concentrated growth factors（ CGF） gel,and to clarify the difference among different layers of CGF. Methods： Venous blood samples were collected from 6 healthy volunteers to prepare CGF. The distribution,integrated optical density（ IOD） and average optical density（ AOD） of TGF-β1 and VEGF in CGF gel and red blood cell（ RBC） layer were measured using immunohistochemistry. The concentrations of TGF-β1 and VEGF in the supernatant serum at baseline and the CGF releasate after 1 day were evaluated with enzyme-linked immunosorbent assays. Results： Abundant TGF-β1 and VEGF were concentrated in CGF gel. However,only a little could be found in polykaryocytes and sporadic platelets in RBC layer. Platelets and leukocytes were concentrated in between the two layers with high expression of TGF-β1. The concentrations of TGF-β1 and VEGF in the CGF releasate（ 55 236. 78 ± 3 686. 34）,（ 610. 99 ± 148. 81） ng / L were significantly higher than those in the supernatant serum（ 20 710. 20 ± 4 523. 14）,（ 335. 20 ± 51. 69） ng / L（ P〈0. 001）.Conclusion： CGF contains high quantities of TGF-β1 that can promote new bone formation and tissue healing. We suggest that CGF gel should be used right after being prepared. Supernatant serum and the area between CGF gel and RBC layer could also be mixed with bone substitute materials.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2016年第5期860-865,共6页
Journal of Peking University:Health Sciences
基金
国家临床重点专科建设项目(2011)
教育部留学回国人员科研启动基金(2012-940)资助~~
关键词
浓缩生长因子
转化生长因子Β1
血管内皮生长因子
骨生成
Concentrated growth factors
Transforming growth factor beta1
Vascular endothelial growth factors
Osteogenesis
