摘要
目的:探讨在体外受精-胚胎移植(in-vitro fertilization-embryo transfer,IVF-ET)过程中,卵裂期胚胎冷冻10年后解冻复苏再移植成功妊娠的可能性。方法:1例患者卵裂期胚胎冷冻10年后解冻复苏并行囊胚培养,获得囊胚后再次冷冻1年后复苏,行囊胚移植。结果:该患者移植后顺利获得临床妊娠,并于孕38周剖宫产一健康男婴。结论:长期保存的胚胎解冻后重新冷冻对最终获得妊娠依然有价值;冷冻胚胎囊胚培养有助于提高卵裂期胚胎冷冻复苏后的利用价值及提高胚胎着床率。
Objective： To investigate the possibility of achieving a pregnancy in frozen-thawed transfer using cleavage stage embryos frozen for 10 years in in-vitro fertilization-embryo transfer （IVF-ET）. Methods： Cleavage stage embryos from one patient were frozen for 10 years and thawed for blastocyst culture. The resulting blastocysts were refrozen for 1 year and thawed for embryo transfer. Results： The patient achieved a clinical pregnancy following transfer and was delivered a healthy baby boy at 38th gestational week in uterine- incision. Conclusion： Refreezing the thawed embryos of long-term storage is still of value in achieving a pregnancy. Blastocyst culture following frozen-thawed process is helpful to improve the use value and implantation rates of thawed cleavage stage embryos.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2016年第10期864-867,共4页
Reproduction and Contraception
关键词
胚胎玻璃化冷冻
冷冻胚胎复苏
囊胚培养
cryopreservation of embryonic by vitrification
frozen embryos resuscitation
blastocyst culture
