摘要
目的采用蛋白酶消化透明带和激光打孔的方法增加胚胎着床的机会,获得临床妊娠。方法对具有透明带增厚(≥15μm);年龄≥35岁;前次胚胎种植失败;胚胎发育延迟及基础FSH≥12mIU/ml共141周期及105个冻融移植(FET)周期的胚胎,予10IU/mlPronase溶液消化或透明带激光打孔后移植。结果68例获临床妊娠,其中22例妊娠来自FET后。2例3胎妊娠,16例双胎妊娠,50例单胎妊娠。结论两种透明带辅助孵化的方法均获得临床妊娠,是方便且实用的辅助生殖技术。
Objective： To evaluate the effectiveness after laser - assisted hatching of the zona pellucida from fresh or frozen - thawed cleavage stage embryos. Methods： 141 patients underwent IVF/ICSI with transfer of fresh cleavage stage embryos and 105 patients underwent transfer of frozen - thawed embryos used the enzymatic treatment of zona pellucida or laser - assisted hatching between October 2003 and August 2005. The main patient indications for assisted hatching included ： the thickness of embryo zona pellucida ≥15 μm ; patient age ≥-35; implantation failure in previous cycle; FSH level on day 3 ≥ 12 mlU/ml; and poor quality embryo transfer and frozen - thawed transfer. Embryos of patients belonged to such indications would be performed assisted hatching before transfer. Results： A total of 246 embryo transfer cycles were enrolled. 68 clinical pregnancies occurred, and 22 pregnancies followed FET. There were 2 triplets, 16 twins and 50 singletons, respectively. Conclusion： The two methods in assisted hatching successful obtain clinical pregnancy, and could be applied conveniently as the new assisted reproductive technologies.
出处
《中国妇幼保健》
CAS
北大核心
2007年第12期1640-1642,共3页
Maternal and Child Health Care of China
基金
安徽省教育厅自然科学基金项目资助(编号:2004kj206)
关键词
辅助孵化
激光
胚胎
透明带
妊娠
Assisted hatching
Laser
Embryos
Zona pellucida
Pregnancy
