摘要
目的探讨低密度脂蛋白受体(LDLR)rs688基因多态性与缺血性脑血管病(ICVD)发生的相关性。方法分别检测264例ICVD患者及180例对照组LDLR的rs688基因多态性、血清LDL、总胆固醇(TC)和Toll样受体2(TLR2)水平。结果 LDLR rs688基因型分布和T等位基因频率,差异有统计学意义(P<0.05)。TT型和总胆固醇型血清LDL、TC水平高于CC基因型,差异有统计学意义(P<0.05),但TT型和总胆固醇型比较,差异无统计学意义(P>0.05)。ICVD组患者的血清LDL、TC、TLR2水平高于对照组,差异有统计学意义(P<0.01)。相关分析结果显示,ICVD组患者血清TLR2与血清LDL水平呈正相关(r=0.801,P<0.05)。结论高浓度LDL、总胆固醇血症是导致ICVD发生的独立危险因素。而LDLR rs688的T等位基因是ICVD发病的易感基因,该基因突变可能通过上调血清LDL、TC、TLR2水平进而介导ICVD的发生。TLR2与血清LDL之间存在的正反馈调节机制的过度活化,可能是介导ICVD发生的重要因素。
Objective To investigate the relationship between low-density lipoprotein receptor（LDLR） gene rs688 polymorphism with ischemic cerebrovascular disease（ICVD）. Methods The LDLR gene rs688 polymorphism for 264 ICVD patients（ICVD group） and 180 healthy people（control group） were detected by PCR and direct nucleotide sequencing analysis. Meanwhile, the serum levels of LDL, TC, and Toll-like receptor 2（TLR2） were detected in all the groups. Results The genotype distribution of LDLR rs688 and the frequency of T allele had significant differences between the ICVD and the control groups（P〉0.05）. Serum LDL and TC levels in the patients with CC genotype were obviously lower than those in the patients with TT and TC genotypes（P〈0.05）. But serum LDL and TC levels had no significant differences between the patients with TT and TC genotypes（P〈0.05）. Serum LDL, TC and TLR2 levels in the ICVD group were significantly higher than those in the control group（P〈0.01）. Meanwhile, correlation analysis showed that serum TLR2 level was positively correlated with LDL level in the ICVD group（r = 0.801, P〈0.05）. Conclusions High levels of serum LDL and TC are independent risk factors for ICVD. T allele of LDLR rs688 is a susceptible gene of ICVD. The polymorphism of LDLR gene rs688 is a genetic risk factor of elevating the serum levels of LDL and TC. High level of serum TLR2 is closely associated with occurrence of ICVD.
出处
《中国现代医学杂志》
CAS
北大核心
2016年第19期32-36,共5页
China Journal of Modern Medicine
基金
辽宁省自然科学基金(No:201102240)
关键词
缺血性脑血管病
低密度脂蛋白受体
基因多态性
low-density lipoprotein receptor
ischemic cerebrovascular disease
gene polymorphism
