摘要
目的:观察贞芪扶正胶囊对再生障碍性贫血(AA)模型大鼠重组人红细胞生成素(EPO),CD34^+细胞,白细胞介素-2(IL-2),白细胞介素-11(IL^(-1)1)的影响,探讨其相关的作用机制。方法:按随机数字将60只清洁级Wistar大鼠分为6组,分别为正常组、模型组、贞芪扶正胶囊低、中、高剂量组(5,10,20 g·kg^(-1))及阳性药组(司坦唑醇混悬液,0.004 g·kg^(-1)),除正常组外,其余均釆用5-氟尿嘧啶(5-FU)与马利兰联合建立大鼠AA模型。模型成功后,给药组给予相应药物给药,阳性药组给予司坦唑醇混悬液,正常组与模型组给予相同体积的生理盐水,连续ig 30 d,以外周血细胞计数,骨髓单个核细胞(BMNC)计数,酶联免疫吸附测定(ELISA)法检测IL-2,IL^(-1)1,EPO,肿瘤坏死因子-α(TNF-α)水平,CD34^+抗原和Fas抗原检测为主要观察指标综合评价贞芪扶正胶囊的干预效果。结果:与正常组比较,模型组的外周血白细胞(WBC),血小板(PLT),红细胞(RBC)及血红蛋白(Hb),BMNC,IL^(-1)1,CD3^+T细胞比例,CD3^+CD4^+T细胞比例,CD34^+抗原荧光量均明显降低,IL-2,EPO,TNF-α,Fas抗原荧光量明显升高(P<0.01);与模型组比较,司坦唑醇组、贞芪扶正胶囊高剂量组的WBC,RBC,PLT,Hb,BMNC均有所升高(P<0.05,P<0.01),贞芪扶正胶囊中、高剂量组的IL^(-1)1,CD3^+T细胞,CD3^+CD4^+T细胞比例,CD34^+抗原荧光量升高,IL-2,EPO,TNF-α,Fas抗原荧光量降低降低(P<0.05,P<0.01);与司坦唑醇组比较,贞芪扶正胶囊中、高剂量组的WBC,CD3^+T细胞比例,CD3^+CD4^+T细胞比例,IL^(-1)1,CD34^+抗原荧光量较高(P<0.05),IL-2,TNF-α,Fas抗原荧光量较低(P<0.05)。结论:贞芪扶正胶囊能够改善AA大鼠外周血细胞状况、骨髓造血组织功能的恢复,增强免疫功能,可能与调控因子EPO,IL-2,IL^(-1)1水平和CD34^+细胞密切相关。
Objective： To observe the effect of Zhenqi Fuzheng capsule on erythrogenin（ EPO）,CD34^＋cells,interleukin-2（ IL-2）,IL-11 in aplastic anemia rats. Method： Totally 60 clean-grade Wistar rats were randomly divided into 6 groups： normal group,model group,Zhenqi Fuzheng capsule low,middle and highdose groups（ 5,10,20 g·kg^-1） and positive drug group（ stanozolol suspension,0. 004 g·kg^-1）. Except for the normal group,the other groups were given 5-fluorouracil（ 5-FU） combined with Maryland to establish aplastic anemia（ AA） rats model. After successful modeling,Zhenqi Fuzheng capsule groups were given Zhenqi Fuzheng capsule,the positive drug group was given Stanozolol suspension,and the normal group and the model group were given the same volume of normal saline,ig,for 30 days in a row. Peripheral blood cells and bone marrow monouclear cells（ BMNCs） were counted. EPO,IL-2,IL-11,and tumor necrosis factor-α（ TNF-α） were detected by ELISA,with CD34^＋antigens and Fas antigens as the main index for the comprehensive evaluation on the intervention effect of Zhenqi Fuzheng capsule. Result： Compared with the normal group,WBC,RBC,PLT,Hb,BMNC,IL-11,proportion of CD3^＋T cells,proportion of CD3^＋CD4^T cells,CD34^＋antigen fluorescent volume of the model group were significantly lower,IL-2,EPO,TNF-α,Fas antigen fluorescent quantity increased significantly（ P〈0. 01）. Compared with the model group,WBC,RBC,PLT,Hb and BMNC cell count in the Stanozolol group,and Zhenqi Fuzheng capsule middle-dose and high-dose groups were increased（ P〈0. 05,P〈0. 01）,IL-11,CD3^＋T cells,proportion of CD3^＋CD4^＋T cells,CD34^＋antigen fluorescent quantity in Zhenqi Fuzheng capsule middle-dose and high-dose groups increased,IL-2,EPO,TNF-α,Fas antigen fluorescence decreased（ P〈0. 05,P〈0. 01）. Compared with the Stanozolol group,WBC,proportion of CD3^＋T cells,CD3^＋,CD4^＋T cell percentage,IL-11,CD34^＋antigen fluorescence quantity in Zhenqi Fuzheng capsule middle-dose and hig
作者
刘现辉
郭晓娜
LIU Xian-hui GUO Xiao-na(Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China Medical School, Huanghe Science and Technology College, Zhengzhou 450063, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第20期143-147,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省中管局重点专科(学科)学术带头人培养项目(121PCXTD510)
