摘要
目的对比观察电针溃疡性结肠炎大鼠上巨虚、足三里、下巨虚、阳陵泉等穴后对结肠组织白介素-1β(IL-1β)及烟碱型乙酰胆碱受体a7信使核糖核酸(nchRa7mRNA)表达的影响,探讨大肠下合穴上巨虚治疗对应腑病是否存在相对特异性。方法将70只健康SD大鼠随机分为空白组、模型组、上巨虚组、足三里组、下巨虚组、阳陵泉组及承筋组,每组10只,雌雄各半。除空白组外均采用2-4-6三硝基苯磺酸/乙醇溶液灌肠诱导建立大鼠溃疡性结肠炎模型,造模成功并治疗10 d后,肉眼观察大鼠结肠黏膜溃疡及炎症情况,ELISA法检测大鼠结肠组织中IL-1b含量,RT-PCR检测nAchRa7mRNA的表达。结果与模型组比,各穴位组结肠损伤有不同程度好转,组织IL-1β含量明显偏低而n ACh Ra7m RNA表达均较高(P<0.05,P<0.01),且上巨虚组、足三里组结肠溃疡评分亦较低(P<0.05,P<0.01);与上巨虚组比,其他4个穴位组结肠n AChRa7mRNA表达均较低(P<0.01),而下巨虚组、阳陵泉组、承筋组结肠溃疡及炎症损伤较严重,结肠溃疡评分及IL-1b含量均偏高(P<0.05,P<0.01)。结论电针治疗溃疡性结肠炎的机制可能是通过影响IL-1β、n Ach Ra7m RNA等调节异常的免疫功能,改善结肠黏膜损伤等来实现的;上巨虚治疗溃疡性结肠炎的总体效应优于足三里、下巨虚、阳陵泉及承筋穴,说明上巨虚穴与对应大肠腑之间存在一定的相对特异性。
Objective To compare the effects of electroacupuncture at points Shangjuxu（ST37）,Zusanli（ST36）,Xiajuxu（ST39） and Yanglingquan（GB34） on colonic expressions of interleukin-1β（IL-1β） and nicotinic acetylcholine receptor a7 m RNA（n Ach Ra7m RNA） in ulcerative colitis rats and investigate if large intestine lower He-Sea point Shangjuxu has relative specificity to fu organ diseases.Method Seventy healthy SD rats were randomized into blank,model,Shangjuxu,Zusanli,Xiajuxu,Yanglingquan and Chengjin groups,10 rats,half male and half female,each.A rat model of ulcerative colitis was made by induction of 2-4-6 three nitrobenzene sulfonic acid/ethanol solution enema in every group except the blank group.After successful model making and ten days of treatment,rat colonic mucosal ulcers and inflammation were observed macroscopically,colonic IL-1β content was measured by ELISA and the expression of n Ach Ra7m RNA was determined by RT-PCR.Result Compared with the model group,colonic lesions were reduced in varying degrees,colonic IL-1β content was significantly lower and the expression of n Ach Ra7m RNA was higher in every acupoint group（P〈0.05,P〈0.01）;the colonic ulcer score was lower in the Shangjuxu and Zusanli groups（P〈0.05,P〈0.01）.Compared with the Shangjuxu group,colonic expression of n Ach Ra7m RNA was lower in the other four acupoint groups（P〈0.01）;colonic mucosal ulcers and inflammatory lesions were more severe and the colonic ulcer score and the IL-1β content were higher in the Xiajuxu,Yanglingquan and Chengjin groups（P〈0.05,P〈0.01）.Conclusion The mechanism of electroacupuncture treatment for ulcerative colitis may be that it regulates abnormal immunologic function by modulating IL-1b and n Ach Ra7m RNA and reduces mucosal lesions.The overall therapeutic effect of Shangjuxu is better than those of Zusanli,Xiajuxu,Yanglingquan and Chengjin,indicating that Shangjuxu has relative specificity to fu organ large intestine.
作者
易细芹
张泓
凌希
吴金峰
艾坤
邓石峰
YI Xi-qin ZHANG Hong LING Xi WU Jin-feng AI Kun DENG Shi-feng(Hunan University of Traditional Chinese Medicine School of Acupuncture and Massage, Changsha 410208,China)
出处
《上海针灸杂志》
2016年第10期1251-1255,共5页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金项目(81173327/H2718)
关键词
电针
穴
下合
结肠炎
溃疡性
大鼠
IL-1B
nAchRa7mRNA
合治内府
Electroacupuncture
Point
lower He-Sea
Colitis
ulcerative
Rats
IL-1b
nAchRa7mRNA
Lower He-Sea point for fu organ diseases
