摘要
目的探讨中国汉族人群基质金属蛋白酶-3（MMP-3）基因多态性与无症状性颈动脉狭窄的关系。方法选择2011年1月至2013年12月来院体检的425例年龄≥60岁健康者为研究对象,经颈动脉超声检测,其中颈动脉狭窄（≥50%）65例,无颈动脉狭窄360例;并采用聚合酶链反应-限制性酶切片段长度多态性方法,分析与比较两组人群MMP-3基因启动子区-1171位点的5A/6A多态性。结果颈动脉狭窄与无狭窄人群基因型分布均符合Hardy-Weinberg平衡（均P〉0.05）;两组人群MMP-3 5A/6A基因型和5A等位基因分布比较,差异均有统计学意义（均P〈0.05）。经logistic回归模型分析,校正多项血管危险因素后,5A/6A或5A/5A基因型是无症状性颈动脉狭窄的独立危险因素（OR=1.98,95%CI：1.05~3.72）。结论 MMP-3基因启动子区-1171位点的5A/6A多态性与无症状性颈动脉狭窄的发生可能相关。
Objective To investigate the relationship between matrix metalloproteinase-3 （MMP-3） -1171 5A/6A polymorphism and the prevalence of asymptomatic carotid artery stenosis in Han Chinese population. Methods Total 425 healthy subjects aged ≥60 years were enrolled in this study from January 2011 to December 2013. The genotypes of MMP-3 promoter polymorphism （-1171 5A/6A） were determined using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, Results Among 425 subjects 65 showed evidence of asymptomatic carotid artery stenosis. The genotype distributions of the asymptomatic carotid artery stenosis group and the non-stenosis group were both in Hardy-Weinberg equilibrium （P 〉0.05）. The frequencies of MMP-3 5A/6A＋5A/5A genotype and 5A allele in the asymptomatic carotid artery stenosis group was significantly higher than those in non-stenosis group（P〈0.05）. After adjustment for vascular risk factors, multivariate logistic regression analysis showed that the MMP-3 5A/6A＋5A/5A genotype was an independent risk factor for asymptomatic carotid artery stenosis （OR=1.98, 95%CI： 1.05-3.72）. Conclusion The MMP-3 （-1171 5A/6A） polymorphism may contribute to susceptibility of asymptomatic carotid artery stenosis.
出处
《浙江医学》
CAS
2016年第18期1494-1497,共4页
Zhejiang Medical Journal
基金
浙江省温州市科技计划项目(Y20140531)
关键词
基质金属蛋白酶-3
基因多态性
无症状性颈动脉狭窄
Matrix metalloproteinase-3 Polymorphism asymptomatic Asymptomatic carotid antery stenosis
