摘要
目的:探讨IL-32在重症肺炎患者血浆中表达的临床意义及其机制。方法:选取2014年3月至2016年3月在金华市第二医院住院的肺炎患者120例,将肺炎入选病例分为普通肺炎组(72例)、重症肺炎组(48例)。同时选取50例健康人作为对照组。采用酶联免疫吸附法检测血清中白介素-6(IL-6)、白介素-8(IL-8)、白介素-10(IL-10)、白介素-32(IL-32)和肿瘤坏死因子-α(TNF-α)的表达。结果:与正常对照组相比,普通肺炎组和重症肺炎组IL-6、IL-8、IL-10、TNF-α和IL-32表达水平明显升高,差异有统计学意义(P﹤0.05);重症肺炎组IL-6、IL-8、IL-10、TNF-α和IL-32表达水平明显高于普通肺炎组,差异有统计学意义(P﹤0.05);IL-6(r=0.86,P﹤0.05)、IL-8(r=0.77,P﹤0.05)、IL-10(r=0.89,P﹤0.05)、TNF-α(r=0.82,P﹤0.05)与IL-32的表达呈正相关。结论:IL-32与重症肺炎的病情程度密切相关,可能是重症肺炎发病机制中的重要参与者。
AIM： To investigate the clinical significance and mechanism of IL-32 in plasma of patients with severe pneumonia. METHODS： 120 patients with pneumonia admitted to the second hospital of Jinhua from March 2014 to March 2016 were divided into common pneumonia group（ 72 cases）and severe pneumonia group（ 48 cases）. And another 50 healthy people were selected as the control group. Enzyme linked immunosorbent assay was used for detecting the expression of serum interleukin-6（ IL-6）,interleukin-8（ IL-8）,interleukin 10（ IL-10）,interleukin-32（ IL-32） and tumor necrosis factor alpha（ TNF-α）. RESULTS： Compared with the control group,the expression of IL-8,IL-10,TNF-α,IL-32 and IL-6 in the common pneumonia group and the severe pneumonia group were significantly higher（ P ﹤0. 05）. The expression of IL-32,TNF-α,IL-8,IL-10 and IL-6 in the severe pneumonia group were significantly higher than those in the common pneumonia group（ P ﹤0. 05）. The expression of IL-6（ r = 0. 86,P ﹤0. 05）,IL-8（ r= 0. 77,P ﹤0. 05）,（ r = 0. 89,P ﹤0. 05）,IL-10 and TNF-α（ r = 0. 82,P ﹤0. 05） were positively correlated with the expression of IL-32. CONCLUSION： IL-32 is closely related to severe pneumonia,and may be an important participant in the pathogenesis of severe pneumonia.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2016年第9期1024-1027,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省医药卫生科技计划项目(2015KYA010)
