摘要
目的:了解鲍曼不动杆菌整合子分布情况,探讨发现的整合子与其耐药性的关系。方法:分离2014—2015年重症监护室(ICU)多重耐药鲍曼不动杆菌,用VITEK2全自动微生物鉴定系统进行菌种鉴定及药敏检测,聚合酶链反应(PCR)检测整合子可变区。结果:鲍曼不动杆菌对ICU常用药物除头孢哌酮舒巴坦外耐药率均超过50%,整合子结构的检出率为82.81%,可变区包含arr-3+aacA4和aacA4+catB8+aadA1,属于两种Ⅰ类整合子。结论:ICU的多重耐药鲍曼不动杆菌广泛存在Ⅰ类整合子结构,介导对氨基糖苷类、氯霉素和利福平耐药,对ICU多重耐药鲍曼不动杆菌的耐药基因有待进一步检测。
OBJECTIVE To investigate distribution of integron of Acinetobacter baumanii（Ab）,analyze correlation between integron and drug resistance.METHODS Multi-drug resistant Acinetobacter baumanii was isolated from ICU from2014 to2015.VITEK2 full automatic bio-identification system was used to detect bacterial species and drug sensitivity.Variable regions of integron were detected by PCR.RESULTS Drug resistance rates of Acinetobacter baumanii were higher than50% except cefoperazone/sulbactam in commonly used antibiotics in ICU.Positive rate of integron was 82.81%,two types of class I integron were detected in variable region,which were arr-3＋aacA4 and aacA4＋catB8＋aadA1.CONCLUSION Class I integron widely exists in multi-drug resistant Acinetobacter baumanii in ICU,and mediates resistance to aminoglycosides,chloramphenicol and rifampin.Further researches are need to investigate gene mutation which results in high drug resistance in ICU.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第19期1693-1696,共4页
Chinese Journal of Hospital Pharmacy
关键词
鲍曼不动杆菌
I类整合子
耐药基因盒
Acinetobacter baumanii
class I integron
resistance gene cassettes
