摘要
有研究证明,自噬在生物应对多种不良环境过程中发挥着重要作用,但是自噬在细胞应对低温环境过程中的功能研究却甚少。该研究给予He La细胞（人子宫颈癌细胞）不同程度的急性冷刺激,探讨此过程中自噬的发生和功能。将He La细胞培养于37°C（对照组）或经不同程度的低温（10、18、28°C）处理,观察对照组与低温处理组细胞表达自噬标志物EGFP-LC3（enhanced green fluorescent protein-microtubule-associated protein 1A/1B-light chain 3）的变化,以监测自噬小体形成。结果显示,低温处理诱导He La细胞发生不同程度的自噬现象：10°C处理3~6 h的细胞内的自噬小体增多且体积增大,18°C处理的细胞内的自噬小体在3 d时最明显,28°C处理的细胞始终维持在较低水平。研究还发现,He La细胞在自噬减弱消失的同时发生细胞死亡,自噬特异性的化学抑制剂Bafilomycin A1处理可以进一步降低不同程度的低温胁迫下He La细胞的存活率,表明自噬在细胞低温应激过程中发挥着保护作用。Bafilomycin A1处理伴随有细胞内活性氧（reactive oxygen species,ROS）水平增高,提示低温胁迫下自噬可能通过调控细胞内ROS水平来保护细胞。该研究结果表明,自噬在真核细胞冷应激中具有重要作用。
Although it has been reported that autophagy is required for organisms surviving various unfavorable environmental conditions, the detailed role of autophagy under cold stress is still unclear. In this study, we investigated the role of autophagy under cold stress in He La cells（human cervical adenocarcinoma cells）. A putative autophagosome marker EGFP-LC3（enhanced green fluorescent protein-microtubule-associated protein 1A/1B-light chain 3） was used to monitor the process of autophagy in He La cells cultured at 37 °C（control） or cold stress conditions（10, 8, 28 °C）. The results showed that cold stress caused autophagy in He La cells in time- and temperaturedependent manners： substantial autophagosome accumulation was observed during 3-6 h at 10 °C, while the most significant autophagy was detected after 3 d at 18°C, and only very low level of autophagosome formation was observed at 28 °C. Further study showed that He La cells underwent decreased cell viability following the turnover of autophagosome, and Bafilomycin A1 treatment enhanced cold stress-induced decrease of cell viability, indicating a protective
出处
《中国细胞生物学学报》
CAS
CSCD
2016年第9期1077-1083,共7页
Chinese Journal of Cell Biology
基金
国家基金委重点项目(批准号:31130049)
上海市自然科学基金项目(批准号:13ZR1419500)
上海市教委水产高峰学科项目资助的课题~~
