摘要
通过采用微波辐射悬浮聚合法制备了磁性微球,并研究了磁性聚合物微球的结构性能和萃取性能。建立了磁性聚合物微球萃取富集-高效液相色谱-紫外联用测定5种磺胺类药物的分析方法,方法检出限在35～65ng/L范围内。将该方法用于测定猪肉和鸡肉样品中的磺胺类物质,样品中未检出,采用加标浓度为5.0μg/kg和30μg/kg考察,加标回收率分别为78.8%～97.6%和89.8%～108%,RSD为小于8.5%,实现了复杂样品中痕量磺胺类多组分的灵敏和简便分析。
Magnetic polymer microspheres were prepared by suspension polymerization. The structural characteristics and extraction performance of resultant microspheres were studied. A method was devel- oped for the determination of five sulfanilamides by the magnetic microspheres extraction coupled with HPLC - UV. The detection limit of the method was 35 - 65 ng/L. The proposal method was applied to simultaneous determination of trace sulfanilamides in pork and chicken samples with the amount was not detected. The concentrations of each sulfanilamide for the spiked sample solutions were 5.0 and 50.0 μg/kg, respectively. The recoveries obtained from pork and chicken samples ranged 78.8% 97.6%, and 89.8% -108%, respectively. And the relative standard deviation （RSDs） were less than 8.5%. The proposal method based on magnetic solid - phase extraction was sensitive and conven- ient for trace determination of sulfanilamides in complex samples.
出处
《贵州师范大学学报（自然科学版）》
CAS
2016年第5期60-66,共7页
Journal of Guizhou Normal University：Natural Sciences
基金
国家自然科学基金(No.21505115)
贵州省科学技术基金(黔科合LH字[2014]:No.7406)
黔西南州科技局项目(No.2014-30)
关键词
磁固相萃取
磺胺类药物
高效液相色谱
猪肉样
鸡肉样
magnetic solid-phase extraction
sulfanilamides
high performance liquid chromatogra-phy
pork samples
chicken samples
