摘要
以柑橘黄龙病寄主长春花为试材,研究建立长春花的组织培养体系。取长春花幼嫩叶片为外植体,进行外植体的消毒处理及不同激素的配方筛选,建立长春花愈伤组织诱导及增殖培养体系。试验表明外植体消毒处理组合为75%乙醇处理30~45 s,0.1%氯化汞处理3 min;培养基为MS基本培养基,愈伤组织诱导的激素组合:2,4-D1.0 mg·L^(-1)+NAA 1.0 mg·L^(-1)+6-BA 2.0 mg·L^(-1),愈伤组织增殖的激素组合:NAA 1.0 mg·L^(-1)+6-BA 2.0mg·L^(-1),长春花愈伤组织诱导率达到90%以上。建立了长春花愈伤组织诱导及增殖培养体系,对利用转基因工程技术抗病育种从而控制柑橘黄龙病具有重要意义。
In this study,the tissue culture system of Catharathus roseus（ L.） G. was presented. Using the young leaves of C. roseus（ L.） G. as explants,the callus induction and proliferation system of C. roseus（ L.） G. was explored through screening disinfection treatment of explants and different hormone formulation. The results showed the explants sterilization treatment combination was 75%ethanol for 30- 45 s and 0. 1% mercuric chloride for 3 min. The medium was MS,the hormones combination were 2,4- D 1. 0 mg /L^- 1＋ NAA 1. 0 mg L^-1＋ 6- BA 2. 0 mg L^-1 for the callus induction,and the hormones combination were NAA 1. 0 mg L^-1＋ 6- BA2. 0 mg L^-1for callus proliferation. The results showed that the callus induced ratio of C. roseus（ L.） G was higher than 90%. The culture system of callus induction and proliferation was established,which will be important for the resistance breeding using transgenic engineering technology to control citrus huanglongbing disease.
出处
《生物学杂志》
CAS
CSCD
2016年第5期31-33,共3页
Journal of Biology
基金
广东省微生物信号与作物病害防控重点实验室开放基金(MSDC-01)
广东省科技计划项目(广东省农作物病虫害绿色防控技术研究开发中心建设)
广东省农业领域引导项目(2013B020309004)
广东省公益研究与能力建设项目(2014B020203003)
关键词
长春花
愈伤组织
诱导
增殖
Catharathus roseus（L.）G. Don
callus tissue
induction
proliferation
