摘要
为促进毛蚶(Scapharca kagoshimensis)的遗传育种研究及开展其资源的保护工作。本研究用4组微卫星多元PCR体系对5个毛蚶全同胞家系进行了家系鉴定分析。研究表明:4组多元PCR体系中的10个微卫星位点均为高多态性位点,使用1组多元PCR体系的子代模拟鉴定成功率和实际鉴定成功率分别为78%和72%,3组以上多元PCR体系子代的鉴定成功率为100%。研究结果表明,微卫星多元PCR技术能快速、准确地将任意子代鉴定至其所属家系,该技术可用于大批量家系材料分析,具有较好的应用价值。
Ark shell（Scapharca kagoshimensis）is a commercially important but dwindling natural resource bivalve species in the shallow coastal waters of the northwestern Pacific.In order to protect and exploit the resource of the species effectively,the inheritance mode of 10 microsatellite markers,which were pooled into 4 multiplex PCR panels,was investigated in newly hatched S.kagoshimensis larvae from five full-sib families,and the feasibility of these markers for kinship estimation was also examined.The result showed that all of these loci were highly polymorphic.The average polymorphism information content was 0.809.The frequency of null alleles was estimated 9.5%.Two loci（Sk04,Sk05）showed deviation from Mendelian segregation in a family（F3）even though null alleles were considered.Parentage analysis showed that with the most informative multiplex set（Panel III）,the simulated and real assignment success could be 78% and 72%,respectively,and 100% of the offspring were correctly allocated to their parents when three or more multiplex PCR panels were used.Our results showed that the four multiplex PCR panels of microsatellites are possible and can be used for rapid and highly efficient parentage assignment in S.kagoshimensis.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第9期18-23,共6页
Periodical of Ocean University of China
基金
海洋公益性行业科研专项(201205023)
教育部博士点基金项目(20130132110009)资助~~
关键词
毛蚶
微卫星标记
多元PCR
家系鉴定
Scapharca kagoshimensis
microsatellite locus
multiplex PCR
parentage assignment
