摘要
为了促进引进大鳞鲃的苗种扩繁和种质资源保护,在江苏北部地区进行了3龄大鳞鲃的池塘养殖和全人工繁殖.催产药物包括4μg/kg的促黄体释放激素、2 mg/kg的马来酸地欧酮和1.8 mg/kg的绒毛膜促性腺激素,干法人工授精.孵化桶内微流水孵化,水温23~25℃,48~70 h内受精卵破膜.3龄亲鱼的产卵量为2.81×10^4~5.42×10^4,平均授精率为93.2%,平均孵化率为80.6%.从1 d仔鱼养至30 d夏花苗,成活率约为66.1%.野生大鳞鲃引进后,从第1代至第3代个体的生长指标和体色等性状发生变化.第2代(白色)和第3代(红色)的体色变异种数量均小于群体的0.3%,第3代不同体色个体的体质量、体长和头长等均存在显著差异,依次为黑灰色〉黄色〉红色,差异具有统计学意义(P〈0.05).
To promote the seedling propagation and germplasm resources protection of introduced Barbus capito, the complete artificial propagation of 3-year-old Barbus capito in ponds in Northern Jiangsu was conducted. The hormones adopted as oxytocin drugs included 4 txg/kg LRH-A2, 2 mg/kg DOM and 1.8 mg/kg HCG. Artificial dry fertilization was used and fry incubated by incubation barrels with low speed running water. When the water temperature ranged between 23-25 ℃, the embryonic development took 48-70 h. The spawning of 3-year-old individual ranged from 2.81×10^4 to 5.42×10^4, and the fertilization rate and hatching rate were about 93.2% and 80.6% respectively. The surviving rate was about 66.1% when the 1-day- old larvas were cultivated to be the 30-day-old summer fingerlings. After the wild Barbus capito was introduced, the phenotypes such as growth indexes and body color changed since the first generation to the third generation of the artificial propagation. The second generation (white) and third generation (red) had mutants on body color, both of which accounted for less than 0.3% of the group. The body weight, the body length and the head length of third generation appeared significant differences among individuals of three body colors (P 〈 0.05 ), i.e. black grey 〉 yellow 〉 red.
出处
《天津师范大学学报(自然科学版)》
CAS
2016年第5期60-64,共5页
Journal of Tianjin Normal University:Natural Science Edition
基金
江苏省农业科技自主创新资助项目(CX(15)1015)
关键词
大鳞鲃
人工繁殖
养殖
体色变异
Barbus cap ito
artificial propagation
breeding
variation of body color