摘要
以杜鹃兰种子萌发的原球茎为试材,研究不同培养基、不同植物生长调节物质(6-BA、NAA和IBA)、活性炭、温度及光照强度对杜鹃兰原球茎增殖的影响。结果表明:1/2MS培养基为适于原球茎增殖的基本培养基;添加一定浓度的6-BA、NAA和IBA均有利于原球茎的增殖,IBA的浓度为1.0mg·L^(-1)时,原球茎增殖率较高,40d可达170.07%;适量添加活性炭对原球茎增殖有促进作用;温度及光照强度的控制对原球茎增殖是必需的。杜鹃兰原球茎增殖的适宜培养条件为1/2MS+1.0mg·L^(-1) 6-BA+1.0mg·L^(-1) IBA+0.5g·L^(-1)活性炭,温度为15℃,光照强度为2 000lx。
The protocorms from the germination of Cremastra appendiculata seed were used as test materials to investigate the effects of the media,growth regulators（6 BA, NAA and IBA）,active carbon （AC）,temperature and light intensity on protocorms proliferation of C. appendiculata. The results showed that 1/2 MS medium was the appropriate basic medium for protocorms proliferation. Adding 6-BA, NAA and IBA into 1/2 MS medium was beneficial to protocorms proliferation. The proliferation rate reached to 170. 07% after 40 days while adding 1.0 mg ·L^-1 IBA,and adding certain amount of AC could promote the proliferation. In addition,the temperature and light intensity were also necessary to the proliferation. In conclusion,the suitable culture condition for protoeorms proliferation of C. appendiculata was 1/2 MS adding 1.0 mg·L^-16-BA, I. 0 mg ·L^-1 IBA and 0. 5 g ·L^-1 AC with 15 ℃ and 2 000 lx light intensity.
出处
《北方园艺》
CAS
北大核心
2016年第19期124-128,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(81360613)
贵州省高层次创新型人才培养计划资助项目(黔科合人才[2015]4031号)
贵州大学研究生创新基金资助项目(研农2016004)
关键词
杜鹃兰
原球茎
增殖培养
Cremastra appendiculata （D. Don. ） Makino
protocorms
proliferation culture
