摘要
[目的]探讨磷脂酰肌醇3激酶（PI3K）蛋白表达量及蛋白激酶B（AKT）信号通路在过氧化氢（H2O2）诱导的PCI2细胞中的作用及天麻素的干预对其通路的影响。[方法]PCI2细胞随机分为正常对照组，模型组（400μmol／LH2O2），天麻素低、中、高浓度组（0．1、1、10μmol／L天麻素＋400μmol／LH2O2）。咪唑蓝法检测各组细胞活力，Hoechst染色观察各组细胞凋亡情况，比色法检测天冬氨酸蛋白水解酶（Caspase3）、Caspase8及Caspase9活性，western blot分析Bcl-2、Bax及P13K蛋白表达量与AKT磷酸化水平。[结果]与正常对照组比较，模型组中细胞活力下降，细胞凋亡率提高，Caspase3、Caspase8及Caspase9活性提高，Bcl-2及PI3K表达量下降，Bax表达量上升，AKT磷酸化水平降低，差异均具有统计学意义（P〈0．01）；与模型组比较，天麻素低、中、高浓度组细胞活力提高，细胞凋亡率降低，Caspase3、Caspase8及Caspase9活性降低，Bcl—2、P13K蛋白表达量及AKT磷酸化水平提高，天麻素中、高浓度组Bax表达量降低，差异均具有统计学意义（P〈0．01）。[结论]天麻素可通过激活P13K／AKT信号通路，从而抑制H2O2诱导的PC12细胞凋亡。
[Objective] To explore the effect of protein expression of phosphatidylcholine 3 kinase （PI3K） and signaling pathway of protein kinase B （AKT） on PC12 cells induced by Hydrogen peroxide H2O2.[Methods]PC12 ceils were randomly divided into normal control group, model group （400 H2O2mol/L）, low, medium and high gastrodin concentration group （0.1,1,10 μmol/L gastrodin ＋ 400μmol/L H2O2 ）. The cell viability was detected by the Imidazole blue method of the method. Hoechst staining was used to observe the apoptosis of each group, the activity of Caspase 3 ,Caspase 8 and Caspase 9 was detected by the colorimetric method, protein expression of Bcl-2, Bax and PI3K and AKT phosphorylation level were analyzed .by blot western.[Results] Compared with the normal control group, cell viability in the model group decreased, cell apoptosis rate improved, the activity of Caspase 3, Caspase 8 and Caspase 9 increased, Bcl-2 and PI3K expression decreased, Bax expression increased, the phosphorylation level of AKT decreased, the differences were statistically significant （ P 〈0.01）; Compared with the model group, cell viability in the gastrodin low, medium and high concentration groups increased, cell apoptosis rate decreased, the activity of Caspase 3, Caspase 8 and Caspase 9 reduced, the protein expression of Bcl-2, PI3K and the phosphorylation level of AKT increased, Bax expression in the gastrodin medium and high concentration groups decreased, the difference was significant meaning （ P 〈0.01）.[Conclusion] Gastrodin can inhibit apoptosis of PC12 cells induced by H2O2 through activating PI3K/AKT signaling pathway.
出处
《医学临床研究》
CAS
2016年第9期1760-1763,共4页
Journal of Clinical Research
