摘要
目的：建立天地精丸的质量标准。方法：显微鉴别天地精丸中的粉碎药材，采用TLC法对天地精丸中的黄精和女贞子进行薄层鉴别；采用HPLC法测定天麻素的含量。结果：显微方法能鉴别地龙、石菖蒲特征片断；薄层鉴别分离度好，易于识别。天麻素在19．80～158．40μg·ml-1浓度范围内，线性关系良好（r=1．0000），平均加样回收率为98．33％，RSD为0．45％（n=6）。结论：本方法简便、重复性好，可用于天地精丸的质量控制。
Objective： To establish the quality standard for Tiandijing pills. Methods： A microscopic method was used to identify the crush herbs. TLC was performed to identify Rhizoma Polygonati and Fructus Ligustri Lucidi. HPLC was used to determine the con- tent of gastrodine. Results： The microscopy could identify earthworm and Acorus tatarinowii. The identification characteristics by TLC were distinct and highly specific. Gastrodine could be determined in HPLC. The linearity of gastrodine was good within the range of 19.80-158.40 μg .ml -1 （ r = 1. 000 0）. The average recovery of gastrodine was 98.33 % （ RSD = 0.45% , n = 6）. Conclusion： The methods of identification and quantification are simple and reproducible, which can be used for the quality control of Tiandijing pills.
出处
《中国药师》
CAS
2016年第10期1954-1956,共3页
China Pharmacist
基金
湖北省卫生和计划生育委员会项目(编号:2010Z-Z06)
武汉市中青年医学骨干人才项目(编号:[2015]7号)
