摘要
目的:建立欣梦安神颗粒的质量控制方法。方法:采用TLC法对方中的丹参、桑椹、酸枣仁(炒)、落花生枝叶及五味子进行定性鉴别;以HPLC法分别对丹参中的原儿茶醛和酸枣仁(炒)中的斯皮诺素进行含量测定。结果:薄层斑点清晰,分离良好,阴性无干扰。原儿茶醛、斯皮诺素分别在3.972~198.600μg·ml^(-1)(r=0.999 9)、7.070~226.240μg·ml^(-1)(r=0.999 9)范围内呈现良好的线性关系;平均回收率分别为98.46%,RSD=1.18%(n=9)和98.20%,RSD=0.90%(n=9)。结论:本研究建立的质量控制方法准确、操作简便,重复性好,专属性强,可有效用于欣梦安神颗粒的质量控制。
Objective： To establish the quality control methods for Xinmeng Anshen granule. Methods： TLC was used to identify Salviae Miltiorrhizae Radix Et Rhizoma,Mori Fructus, Ziziphi Spinosae Semen（fried） ,Arachis Hypogaea L. and Schisandrae Chinensis Fructus in the granules. HPLC was used to determine the content of protocatechuic aldehyde in Salviae Miltiorrhizae Radix Et Rhizoma and spinosin in Ziziphi Spinosae Semen. Results： The TLC spots were clear and well-separated without any interference from the nega- tive sample. The calibration curves were linear within the range of 3. 972-198. 600 μg . ml-1 （ r = 0.999 9 ） for protocatechuic alde- hyde and 7. 070-226. 240 μg. ml- 1 （ r = 0. 999 9） for spinosin. The average recovery was 98.46% （RSD = 1.18%, n = 9） for proto- catechuic aldehyde and 98.20% （RSD = 0.90%, n = 9） for spinosin. Conclusion： The established quality control methods are accu- rate, simple, repeatable and specific. It can be well used for the quality control of Xinmeng Anshen granule.
出处
《中国药师》
CAS
2016年第10期1950-1954,共5页
China Pharmacist
基金
国家"重大新药创新"科技重大专项(编号:2010ZX09401-302)
